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Synthetic, marine, light-driven, autotroph-heterotroph co-culture system for sustainable ß-caryophyllene production.
Chen, Wenchao; Park, Young-Kyoung; Studená, Lucie; Bell, David; Hapeta, Piotr; Fu, Jing; Nixon, Peter J; Ledesma-Amaro, Rodrigo.
Afiliación
  • Chen W; Oil Crops Research Institute of the Chinese Academy of Agricultural Sciences, Wuhan 430062, PR China; Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK.
  • Park YK; Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK; Université Paris-Saclay, INRAE, AgroParisTech, Micalis Institute, 78350 Jouy-en-Josas, France.
  • Studená L; Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK.
  • Bell D; SynbiCITE Innovation and Knowledge Centre, Imperial College London, London SW7 2AZ, UK.
  • Hapeta P; Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK.
  • Fu J; Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK.
  • Nixon PJ; Department of Life Sciences, Imperial College London, London SW7 2AZ, UK.
  • Ledesma-Amaro R; Department of Bioengineering, Bezos Centre for Sustainable Protein, Microbial Food Hub and Centre for Synthetic Biology, Imperial College London, London SW72AZ, UK. Electronic address: r.ledesma-amaro@imperial.ac.uk.
Bioresour Technol ; 410: 131232, 2024 Oct.
Article en En | MEDLINE | ID: mdl-39117247
ABSTRACT
Applying low-cost substrate is critical for sustainable bioproduction. Co-culture of phototrophic and heterotrophic microorganisms can be a promising solution as they can use CO2 and light as feedstock. This study aimed to create a light-driven consortium using a marine cyanobacterium Synechococcus sp. PCC 7002 and an industrial yeast Yarrowia lipolytica. First, the cyanobacterium was engineered to accumulate and secrete sucrose by regulating the expression of genes involved in sucrose biosynthesis and transport, resulting in 4.0 g/L of sucrose secretion. Then, Yarrowia lipolytica was engineered to efficiently use sucrose and produce ß-caryophyllene that has various industrial applications. Then, co- and sequential-culture were optimized with different induction conditions and media compositions. A maximum ß-caryophyllene yield of 14.1 mg/L was obtained from the co-culture. This study successfully established an artificial light-driven consortium based on a marine cyanobacterium and Y. lipolytica, and provides a foundation for sustainable bioproduction from CO2 and light through co-culture systems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cocultivo / Yarrowia / Synechococcus / Sesquiterpenos Policíclicos / Luz Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cocultivo / Yarrowia / Synechococcus / Sesquiterpenos Policíclicos / Luz Idioma: En Revista: Bioresour Technol Asunto de la revista: ENGENHARIA BIOMEDICA Año: 2024 Tipo del documento: Article País de afiliación: Reino Unido Pais de publicación: Reino Unido