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Development of a 3-dimensional organotypic model with characteristics of peripheral sensory nerves.
Koyanagi, Madoka; Ogido, Ryosuke; Moriya, Akari; Saigo, Mamiko; Ihida, Satoshi; Teranishi, Tomoko; Kawada, Jiro; Katsuno, Tatsuya; Matsubara, Kazuo; Terada, Tomohiro; Yamashita, Akira; Imai, Satoshi.
Afiliación
  • Koyanagi M; Department of Medical Neuropharmacology, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama 640-8156, Japan.
  • Ogido R; Department of Clinical Pharmacology and Therapeutics, Graduate School of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8507, Japan.
  • Moriya A; Department of Clinical Pharmacology and Therapeutics, Faculty of Pharmaceutical Sciences, Kyoto University, Kyoto 606-8507, Japan.
  • Saigo M; Department of Clinical Pharmacology and Therapeutics, Kyoto University Hospital, Kyoto 606-8507, Japan.
  • Ihida S; New Business Promotion Division, Business Development Unit, Panel Semicon Laboratories, Sharp Corporation, Tenri, Nara 632-8567, Japan.
  • Teranishi T; New Business Promotion Division, Business Development Unit, Panel Semicon Laboratories, Sharp Corporation, Tenri, Nara 632-8567, Japan.
  • Kawada J; Jiksak Bioengineering, Inc., Kawasaki, Kanagawa 210-0821, Japan.
  • Katsuno T; Division of Electron Microscopic Study, Center for Anatomical Studies, Graduate School of Medicine, Kyoto University, Kyoto 606-8501, Japan.
  • Matsubara K; School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama 640-8156, Japan.
  • Terada T; Department of Clinical Pharmacology and Therapeutics, Kyoto University Hospital, Kyoto 606-8507, Japan.
  • Yamashita A; Department of Medical Neuropharmacology, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama 640-8156, Japan.
  • Imai S; Department of Medical Neuropharmacology, School of Pharmaceutical Sciences, Wakayama Medical University, Wakayama 640-8156, Japan; Department of Clinical Pharmacology and Therapeutics, Kyoto University Hospital, Kyoto 606-8507, Japan. Electronic address: imais06@wakayama-med.ac.jp.
Cell Rep Methods ; 4(8): 100835, 2024 Aug 19.
Article en En | MEDLINE | ID: mdl-39116883
ABSTRACT
We developed a rat dorsal root ganglion (DRG)-derived sensory nerve organotypic model by culturing DRG explants on an organoid culture device. With this method, a large number of organotypic cultures can be produced simultaneously with high reproducibility simply by seeding DRG explants derived from rat embryos. Unlike previous DRG explant models, this organotypic model consists of a ganglion and an axon bundle with myelinated A fibers, unmyelinated C fibers, and stereo-myelin-forming nodes of Ranvier. The model also exhibits Ca2+ signaling in cell bodies in response to application of chemical stimuli to nerve terminals. Further, axonal transection increases the activating transcription factor 3 mRNA level in ganglia. Axons and myelin are shown to regenerate 14 days following transection. Our sensory organotypic model enables analysis of neuronal excitability in response to pain stimuli and tracking of morphological changes in the axon bundle over weeks.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Axones / Ganglios Espinales / Sistemas Microfisiológicos Límite: Animals Idioma: En Revista: Cell Rep Methods Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Axones / Ganglios Espinales / Sistemas Microfisiológicos Límite: Animals Idioma: En Revista: Cell Rep Methods Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Estados Unidos