Your browser doesn't support javascript.
loading
Nanodisc assembly from bacterial total lipid extracts.
Llewellyn, Trent R; Pimentel, Olivia R C; Lenz, Kiersten D; Montoya, Makaela M; Kubicek-Sutherland, Jessica Z.
Afiliación
  • Llewellyn TR; Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 United States.
  • Pimentel ORC; Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 United States.
  • Lenz KD; Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 United States.
  • Montoya MM; Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 United States.
  • Kubicek-Sutherland JZ; Physical Chemistry and Applied Spectroscopy Group, Chemistry Division, Los Alamos National Laboratory, P.O. Box 1663, Los Alamos, NM 87545 United States. Electronic address: jzk@lanl.gov.
Chem Phys Lipids ; 264: 105425, 2024 Oct.
Article en En | MEDLINE | ID: mdl-39111725
ABSTRACT
Nanodiscs are discoidal lipoproteins that have often been used as vehicles to study membrane proteins in their native configuration. Nanodiscs have been primarily made from synthetic lipids. However, nanodiscs also offer a format by which native lipids can be studied in their natural configuration. Here, we present a method to synthesize nanodiscs from bacterial total lipid extracts using the biothreat agent, Yersinia pestis, as a proof-of-concept. The creation of nanoparticles entirely composed of bacterial lipids supports membrane characterization and vaccine antigen discovery without the inherent safety concerns associated with live bacterial cells of this Tier 1 select agent pathogen.
Asunto(s)
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yersinia pestis / Lípidos Idioma: En Revista: Chem Phys Lipids Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Yersinia pestis / Lípidos Idioma: En Revista: Chem Phys Lipids Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda