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Screening of novel disease genes of sepsis-induced myocardial Disfunction by RNA sequencing and bioinformatics analysis.
Yao, Hanyi; Xiao, Zixi; Liu, Shufang; Gao, Xingjian; Wu, Zehong; Li, Dongping; Yi, Zhangqing; Zhou, Haojie; Zhang, Weizhi.
Afiliación
  • Yao H; Department of Cardiovascular Surgery, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China; Clinical Center for Gene Diagnosis and Therapy, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Xiao Z; Department of Cardiovascular Medicine, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Liu S; Clinical Center for Gene Diagnosis and Therapy, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Gao X; Clinical Center for Gene Diagnosis and Therapy, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Wu Z; Department of Cardiovascular Surgery, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China; Clinical Center for Gene Diagnosis and Therapy, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Li D; Department of Cardiovascular Surgery, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China; Clinical Center for Gene Diagnosis and Therapy, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Yi Z; Department of Cardiovascular Surgery, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China; Clinical Center for Gene Diagnosis and Therapy, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Zhou H; Department of Cardiovascular Surgery, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China.
  • Zhang W; Department of Cardiovascular Surgery, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China; Clinical Center for Gene Diagnosis and Therapy, the Second Xiangya Hospital of Central South University, Changsha, Hunan 410011, China. Electronic address: zhangwz@csu.edu.cn
Genomics ; 116(5): 110911, 2024 Sep.
Article en En | MEDLINE | ID: mdl-39111545
ABSTRACT

BACKGROUND:

There is still a lack of effective treatment for sepsis-induced myocardial dysfunction (SIMD), while the pathogenesis of SIMD still remains largely unexplained.

METHODS:

RNA sequencing results (GSE267388 and GSE79962) were used for cross-species integrative analysis. Bioinformatic analyses were used to delve into function, tissue- and cell- specificity, and interactions of genes. External datasets and qRT-PCR experiments were used for validation. L1000 FWD was used to predict targeted drugs, and 3D structure files were used for molecular docking.

RESULTS:

Based on bioinformatic analyses, ten differentially expressed genes were selected as genes of interest, seven of which were verified to be significantly differential expression. Bucladesine was considered as a potential targeted drug for SIMD, which banded to seven target proteins primarily by forming hydrogen bonds.

CONCLUSION:

It was considered that Cebpd, Timp1, Pnp, Osmr, Tgm2, Cp, and Asb2 were novel disease genes, while bucladesine was a potential therapeutic drug, of SIMD.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sepsis / Biología Computacional Límite: Animals / Humans Idioma: En Revista: Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Sepsis / Biología Computacional Límite: Animals / Humans Idioma: En Revista: Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos