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DNA-PAINT Probe Modifications Support High-Resolution Imaging with Shorter Binding Domains.
Piantanida, Luca; Dickinson, George D; Majikes, Jacob M; Clay, William; Liddle, J Alexander; Andersen, Tim; Hayden, Eric J; Kuang, Wan; Hughes, William L.
Afiliación
  • Piantanida L; Micron School of Materials Science & Engineering, Boise State University, Boise, Idaho 83725, United States.
  • Dickinson GD; Micron School of Materials Science & Engineering, Boise State University, Boise, Idaho 83725, United States.
  • Majikes JM; National Institute of Standards and Technology, Gaithersburg, Maryland 20878, United States.
  • Clay W; Micron School of Materials Science & Engineering, Boise State University, Boise, Idaho 83725, United States.
  • Liddle JA; National Institute of Standards and Technology, Gaithersburg, Maryland 20878, United States.
  • Andersen T; Department of Computer Science, Boise State University, Boise, Idaho 83725, United States.
  • Hayden EJ; Micron School of Materials Science & Engineering, Boise State University, Boise, Idaho 83725, United States.
  • Kuang W; Department of Biological Sciences, Boise State University, Boise, Idaho 83725, United States.
  • Hughes WL; Department of Electrical & Computer Engineering, Boise State University, Boise, Idaho 83725, United States.
ACS Nano ; 18(33): 22369-22377, 2024 Aug 20.
Article en En | MEDLINE | ID: mdl-39109416
ABSTRACT
DNA-based Points Accumulation for Imaging in Nanoscale Topography (DNA-PAINT) is an effective super resolution microscopy technique, and its optimization is key to improve nanoscale detection. The state-of-the-art improvements that are at the base of this optimization have been first routinely validated on DNA nanostructure devices before being tested on biological samples. This allows researchers to finely tune DNA-PAINT imaging features in a more controllable in vitro environment. Dye-labeled oligonucleotide probes with short hybridization domains can expand DNA-PAINT's detection by targeting short nucleotide sequences and improving resolution, speed, and multiplexing. However, developing these probes is challenging as their brief bound state makes them difficult to capture under routine imaging conditions. To extend dwell binding times and promote duplex stability, we introduced structural and chemical modifications to our imager probes. The modifications included mini-hairpins and/or Bridged Nucleic Acids (BNA); both of which increase the thermomechanical stability of a DNA duplex. Using this approach we demonstrate DNA-PAINT imaging with approximately 5 nm resolution using a 4-nucleotide hybridization domain that is 43% shorter than previously reported probes. Imager probes with such short hybridization domains are key for improving detection on DNA nanostructure devices because they have the capability to target a larger number of binding domains per localization unit. This is essential for metrology applications such as Nucleic Acid Memory (NAM) where the information density is dependent on the binding site length. The selected imager probes reported here present imaging resolution equivalent to current state-of-the-art DNA-PAINT probes, creating a strategy to image shorter DNA domains for nanoscience and nanotechnology alike.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Idioma: En Revista: ACS Nano Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ADN Idioma: En Revista: ACS Nano Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos