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Disrupted neuregulin 1-ErbB4 signaling: Consequences of prenatal morphine exposure in rat pups and molecular gateway to neurological impairment.
Khayat, Samira; Fanaei, Hamed; Hafezinouri, Hamid; Ghanbarzehi, Abdolhakim; Parsi-Moud, Abolfazl; Mirzaei, Ilia.
Afiliación
  • Khayat S; Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
  • Fanaei H; Department of Midwifery, School of Nursing and Midwifery, Zahedan University of Medical Sciences, Zahedan, Iran.
  • Hafezinouri H; Pregnancy Health Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
  • Ghanbarzehi A; Department of Physiology, School of Medicine, Zahedan University of Medical Sciences, Zahedan, Iran.
  • Parsi-Moud A; Laboratory Animal Research Center, Zahedan University of Medical Sciences, Zahedan, Iran.
  • Mirzaei I; Department of Neuroscience, School of Advanced Technologies in Medicine, Iran University of Medical Sciences, Tehran, Iran.
Toxicol Rep ; 13: 101687, 2024 Dec.
Article en En | MEDLINE | ID: mdl-39109071
ABSTRACT

Objective:

Morphine exposure during pregnancy has detrimental effects on both the mother and her offspring, both during and after childbirth. This study aimed to investigate the impact of prenatal morphine exposure on rat pups and dams, specifically focusing on changes in Neuregulin-1 (Nrg-1)/ErbB4 gene expression, inflammation, and brain-derived neurotrophic factor (BDNF) levels. Materials and

methods:

Twenty female rats were randomized into two experimental groups1-Morphine Group Dams received morphine throughout pregnancy. 2-Control Group Dams received no interventions.At the end of gestation, blood samples were collected from the dams. Subsequently, dams and their pups underwent tissue collection from the cortical area of the brain to evaluate the following parameters Interleukin-6 (IL-6), Interleukin-10 (IL-10), total antioxidant capacity (TAC), Malondialdehyde (MDA), and Brain-derived neurotrophic factor (BDNF).Additionally, RNA was extracted from the pup's cortical brain tissue for the assessment of gene expression levels of Neuregulin-1 (NRG-1) and ErbB-4 using quantitative real-time polymerase chain reaction (qrt-PCR).

Results:

The molecular investigation revealed a decrease in NRG-1 and ErbB-4 expressions in the brain cortex of offspring exposed to morphine during prenatal development. Additionally, the levels of IL-6 and IL-10 in both the serum and brain of both the mothers and their offspring in the morphine group were significantly higher compared to the control group. The morphine-exposed group also exhibited significantly lower levels of TAC and higher levels of MDA, indicating increased oxidative stress. Furthermore, the levels of BDNF in the morphine group were significantly lower compared to the control group.

Conclusion:

Prenatal morphine exposure in rats has detrimental effects on both the dams and their offspring. This study demonstrates that prenatal morphine exposure disrupts critical molecular pathways involved in neurodevelopment, inflammation, oxidative stress, and neurotrophic signaling. These findings suggest that prenatal morphine exposure can have long-lasting consequences for the offspring, potentially contributing to neurodevelopmental disorders and other health issues later in life.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Toxicol Rep Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Toxicol Rep Año: 2024 Tipo del documento: Article Pais de publicación: Irlanda