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Harnessing the power of Neobacillus niacini AUMC-B524 for silver oxide nanoparticle synthesis: optimization, characterization, and bioactivity exploration.
El-Sapagh, Shimaa H; El-Zawawy, Nessma A; Elshobary, Mostafa E; Alquraishi, Mohammed; Zabed, Hossain M; Nouh, Hoda S.
Afiliación
  • El-Sapagh SH; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
  • El-Zawawy NA; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
  • Elshobary ME; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt. mostafa_elshobary@science.tanta.edu.eg.
  • Alquraishi M; Department of Community Health Sciences, College of Applied Medical Sciences, King Saud University, 11421, Riyadh, Saudi Arabia.
  • Zabed HM; School of Life Sciences, Guangzhou University, Guangzhou, 510006, Guangdong, China.
  • Nouh HS; Department of Botany and Microbiology, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Microb Cell Fact ; 23(1): 220, 2024 Aug 06.
Article en En | MEDLINE | ID: mdl-39107838
ABSTRACT

BACKGROUND:

Biotechnology provides a cost-effective way to produce nanomaterials such as silver oxide nanoparticles (Ag2ONPs), which have emerged as versatile entities with diverse applications. This study investigated the ability of endophytic bacteria to biosynthesize Ag2ONPs.

RESULTS:

A novel endophytic bacterial strain, Neobacillus niacini AUMC-B524, was isolated from Lycium shawii Roem. & Schult leaves and used to synthesize Ag2ONPS extracellularly. Plackett-Burman design and response surface approach was carried out to optimize the biosynthesis of Ag2ONPs (Bio-Ag2ONPs). Comprehensive characterization techniques, including UV-vis spectral analysis, Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, dynamic light scattering analysis, Raman microscopy, and energy dispersive X-ray analysis, confirmed the precise composition of the Ag2ONPS. Bio-Ag2ONPs were effective against multidrug-resistant wound pathogens, with minimum inhibitory concentrations (1-25 µg mL-1). Notably, Bio-Ag2ONPs demonstrated no cytotoxic effects on human skin fibroblasts (HSF) in vitro, while effectively suppressing the proliferation of human epidermoid skin carcinoma (A-431) cells, inducing apoptosis and modulating the key apoptotic genes including Bcl-2 associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), Caspase-3 (Cas-3), and guardian of the genome (P53).

CONCLUSIONS:

These findings highlight the therapeutic potential of Bio-Ag2ONPs synthesized by endophytic N. niacini AUMC-B524, underscoring their antibacterial efficacy, anticancer activity, and biocompatibility, paving the way for novel therapeutic strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Plata / Nanopartículas del Metal / Antibacterianos Límite: Humans Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Compuestos de Plata / Nanopartículas del Metal / Antibacterianos Límite: Humans Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Reino Unido