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Solution to a case involving the interpretation of trace degraded DNA mixtures.
Chen, Ji; Chen, Anqi; Tao, Ruiyang; Zhu, Ruxin; Zhang, Han; You, Xuechun; Li, Chengtao; Zhang, Suhua.
Afiliación
  • Chen J; Institute of Forensic Science, Fudan University, Shanghai, 200032, PR China.
  • Chen A; Institute of Forensic Science, Fudan University, Shanghai, 200032, PR China.
  • Tao R; Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Ministry of Justice, Academy of Forensic Science, Shanghai, 200063, PR China.
  • Zhu R; Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Ministry of Justice, Academy of Forensic Science, Shanghai, 200063, PR China.
  • Zhang H; Department of Forensic Medicine, Guizhou Medical University, Guiyang, 550004, PR China.
  • You X; Institute of Forensic Science, Fudan University, Shanghai, 200032, PR China.
  • Li C; Institute of Forensic Science, Fudan University, Shanghai, 200032, PR China. lichengtao@fudan.edu.cn.
  • Zhang S; Shanghai Key Laboratory of Forensic Medicine, Shanghai Forensic Service Platform, Ministry of Justice, Academy of Forensic Science, Shanghai, 200063, PR China. lichengtao@fudan.edu.cn.
Int J Legal Med ; 2024 Aug 07.
Article en En | MEDLINE | ID: mdl-39107630
ABSTRACT
DNA mixture analysis poses a significant challenge in forensic genetics, particularly when dealing with degraded and trace amount DNA samples. Multi-SNPs (MNPs) are genetic markers similar to microhaplotypes but with smaller molecular sizes (< 75 bp), making them theoretically more suitable for analyzing degraded and trace amount samples. In this case report, we investigated a cold case involving a campstool stored for over a decade, aiming to detect and locate the suspect's DNA. We employed both conventional capillary electrophoresis-based short tandem repeat (CE-STR) analysis and next-generation sequencing-based multi-SNP (NGS-MNP) analysis. The typing results and deconvolution of the mixed CE-STR profiles were inconclusive regarding the presence of the suspect's DNA in the mixed samples. However, through NGS-MNP analysis and presence probability calculations, we determined that the suspect's DNA was present in the samples from Sect. 4-1 with a probability of 1-8.41 × 10- 6 (99.999159%). This evidence contradicted the suspect's statement and aided in resolving the case. Our findings demonstrate the significant potential of MNP analysis for examining degraded and trace amount DNA mixtures in forensic investigations.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Legal Med Asunto de la revista: JURISPRUDENCIA Año: 2024 Tipo del documento: Article Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Int J Legal Med Asunto de la revista: JURISPRUDENCIA Año: 2024 Tipo del documento: Article Pais de publicación: Alemania