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Three-Dimensional Electrically Conductive Scaffolds to Culture Cardiac Progenitor Cells.
Ul Haq, Arsalan; Carotenuto, Felicia; De Matteis, Fabio; Di Nardo, Paolo.
Afiliación
  • Ul Haq A; Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy.
  • Carotenuto F; Interdepartmental Research Centre for Regenerative Medicine (CIMER), Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy.
  • De Matteis F; Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy.
  • Di Nardo P; Interdepartmental Research Centre for Regenerative Medicine (CIMER), Department of Clinical Sciences and Translational Medicine, University of Rome "Tor Vergata", Rome, Italy.
Methods Mol Biol ; 2835: 269-275, 2024.
Article en En | MEDLINE | ID: mdl-39105922
ABSTRACT
Three-dimensional (3D) scaffolds provide cell support while improving tissue regeneration through amplified cellular responses between implanted materials and native tissues. So far, highly conductive cardiac, nerve, and muscle tissues have been engineered by culturing stem cells on electrically inert scaffolds. These scaffolds, even though suitable, may not be very useful compared to the results shown by cells when cultured on conductive scaffolds. Noticing the mature phenotype the stem cells develop over time when cultured on conductive scaffolds, scientists have been trying to impart conductivity to traditionally nonconductive scaffolds. One way to achieve this goal is to blend conductive polymers (polyaniline, polypyrrole, PEDOTPSS) with inert biomaterials and produce a 3D scaffold using various fabrication techniques. One such technique is projection micro-stereolithography, which is an additive manufacturing technique. It uses a photosensitive solution blended with conductive polymers and uses visible/UV light to crosslink the solution. 3D scaffolds with complex architectural features down to microscale resolution can be printed with this technique promptly. This chapter reports a protocol to fabricate electrically conductive scaffolds using projection micro-stereolithography.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Técnicas de Cultivo de Célula / Ingeniería de Tejidos / Conductividad Eléctrica / Andamios del Tejido Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Polímeros / Técnicas de Cultivo de Célula / Ingeniería de Tejidos / Conductividad Eléctrica / Andamios del Tejido Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Italia Pais de publicación: Estados Unidos