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Isolation and Characterization of Human Dental Pulp Stem Cells Derived from Dental Pulp of Permanent Teeth.
Adamickova, Adriana; Chomanicova, Nikola; Adamicka, Matus; Valaskova, Simona; Gazova, Andrea; Kyselovic, Jan.
Afiliación
  • Adamickova A; Faculty of Medicine, 5th Department of Internal Medicine, Comenius University Bratislava, Bratislava, Slovakia.
  • Chomanicova N; International Laser Center, Slovak Centre of Scientifc and Technical Information, Bratislava, Slovakia.
  • Adamicka M; Faculty of Medicine, Institute of Medical Biology, Genetics and Clinical Genetics, Comenius University Bratislava, Bratislava, Slovakia.
  • Valaskova S; International Laser Center, Slovak Centre of Scientifc and Technical Information, Bratislava, Slovakia.
  • Gazova A; Faculty of Medicine, Institute of Pharmacology and Clinical Pharmacology, Comenius University Bratislava, Bratislava, Slovakia.
  • Kyselovic J; Faculty of Medicine, 5th Department of Internal Medicine, Comenius University Bratislava, Bratislava, Slovakia.
Methods Mol Biol ; 2835: 49-57, 2024.
Article en En | MEDLINE | ID: mdl-39105905
ABSTRACT
Dental pulp stem cells (DPSCs) are a promising alternative to the source of mesenchymal stem cells (MSCs), as they are readily available in minimally invasive procedures compared to more invasive methods associated with harvesting other MSCs sources. Despite the encouraging pre-clinical outcomes in animal disease models, culture-expanding procedures are needed to obtain a sufficient number of MSCs required for delivery to the damaged site. However, this contributes to increasing regulatory difficulties in translating stem cells and tissue engineering therapy to clinical use. Moreover, discussions continue as to which isolation method is to be preferred when obtaining DPSCs from extracted molars. This protocol describes a simple explant isolation technique of human dental pulp stem cells from the dental pulp of permanent teeth based upon the plastic adherence of MSCs and subsequent outgrowth of cells out of tissue fragments with high efficacy.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Separación Celular / Pulpa Dental / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Separación Celular / Pulpa Dental / Células Madre Mesenquimatosas Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Eslovaquia Pais de publicación: Estados Unidos