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Attenuation of endoplasmic reticulum stress improves invitro growth and subsequent maturation of bovine oocytes.
Islam, Md Nuronnabi; Ebara, Fumio; Kawasaki, Kokoro; Konno, Toshihiro; Tatemoto, Hideki; Yamanaka, Ken-Ichi.
Afiliación
  • Islam MN; Faculty of Agriculture, Saga University, Saga, Japan; The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, Japan; Department of Animal Science, Bangladesh Agricultural University, Mymensingh, Bangladesh.
  • Ebara F; Faculty of Agriculture, Saga University, Saga, Japan; The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, Japan.
  • Kawasaki K; Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan.
  • Konno T; The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, Japan; Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan.
  • Tatemoto H; The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, Japan; Faculty of Agriculture, University of the Ryukyus, Okinawa, Japan.
  • Yamanaka KI; Faculty of Agriculture, Saga University, Saga, Japan; The United Graduate School of Agricultural Science, Kagoshima University, Kagoshima, Japan. Electronic address: kyama@cc.saga-u.ac.jp.
Theriogenology ; 228: 54-63, 2024 Oct 15.
Article en En | MEDLINE | ID: mdl-39096624
ABSTRACT
Endoplasmic reticulum (ER) stress interferes with developmental processes in oocyte maturation and embryo development. Invitro growth (IVG) is associated with low developmental competence, and ER stress during IVG culture may play a role. Therefore, this study aimed to examine the effect of tauroursodeoxycholic acid (TUDCA), an ER stress inhibitor, on the IVG of bovine oocytes to understand the role of ER stress. Oocyte-granulosa cell complexes (OGCs) were collected from early antral follicles (1.5-1.8 mm) and allowed to grow in vitro for 5 days at 38.5 °C in a humidified atmosphere containing 5 % CO2. Basic growth culture medium was supplemented with TUDCA at various concentrations (0, 50, 100, 250, and 500 µM). After IVG, oocyte diameters were similar among groups, but the antrum formation rate tended to be higher in the TUDCA 100 µM group. The mRNA expression levels of ER stress-associated genes (PERK, ATF6, ATF4, CHOP, BAX, IRE1, and XBP1) in OGCs were downregulated in the TUDCA 100 µM group than those in the control group. Moreover, the TUDCA 100 µM group exhibited reduced ROS production with higher GSH levels and improved in vitro-grown oocyte maturation compared with those in the control group. In contrast, no difference in the developmental competence of embryos following invitro fertilization was observed between the control and TUDCA 100 µM groups. These results indicate that ER stress could impair IVG and subsequent maturation rate of bovine oocytes, and TUDCA could alleviate these detrimental effects. These outcomes might improve the quality of oocytes in IVG culture in assisted reproductive technology.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Ácido Tauroquenodesoxicólico / Técnicas de Maduración In Vitro de los Oocitos / Estrés del Retículo Endoplásmico Límite: Animals Idioma: En Revista: Theriogenology Año: 2024 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Oocitos / Ácido Tauroquenodesoxicólico / Técnicas de Maduración In Vitro de los Oocitos / Estrés del Retículo Endoplásmico Límite: Animals Idioma: En Revista: Theriogenology Año: 2024 Tipo del documento: Article País de afiliación: Bangladesh Pais de publicación: Estados Unidos