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Enhancement of astaxanthin accumulation via energy reassignment by removing the flagella of Haematococcus pluvialis.
Hou, Yuyong; Guo, Zhile; Liu, Zhiyong; Yan, Suihao; Cui, Meijie; Chen, Fangjian; Wang, Weijie; Yu, Longjiang; Zhao, Lei.
Afiliación
  • Hou Y; College of Life Science, North China University of Science and Technology, Tangshan, China.
  • Guo Z; Photosynthesis Research Center, Key Laboratory of Photobiology, Institute of Botany, Chinese Academy of Sciences, Beijing, China.
  • Liu Z; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
  • Yan S; National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
  • Cui M; University of Chinese Academy of Sciences, Beijing, China.
  • Chen F; College of Life Science, North China University of Science and Technology, Tangshan, China.
  • Wang W; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
  • Yu L; Key Laboratory of Engineering Biology for Low-carbon Manufacturing, Tianjin Institute of Industrial Biotechnology, Chinese Academy of Sciences, Tianjin, China.
  • Zhao L; National Center of Technology Innovation for Synthetic Biology, Tianjin, China.
Bioresour Bioprocess ; 11(1): 78, 2024 Aug 02.
Article en En | MEDLINE | ID: mdl-39095685
ABSTRACT
Astaxanthin biosynthesis in Haematococcus pluvialis is driven by energy. However, the effect of the flagella-mediated energy-consuming movement process on astaxanthin accumulation has not been well studied. In this study, the profiles of astaxanthin and NADPH contents in combination with the photosynthetic parameters with or without flagella enabled by pH shock were characterized. The results demonstrated that there was no significant alteration in cell morphology, with the exception of the loss of flagella observed in the pH shock treatment group. In contrast, the astaxanthin content in the flagella removal groups was 62.9%, 62.8% and 91.1% higher than that of the control at 4, 8 and 12 h, respectively. Simultaneously, the increased Y(II) and decreased Y(NO) suggest that cells lacking the flagellar movement process may allocate more energy towards astaxanthin biosynthesis. This finding was verified by NADPH analysis, which revealed higher levels in flagella removal cells. These results provide preliminary insights into the underlying mechanism of astaxanthin accumulation enabled by energy reassignment in movement-lacking cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioresour Bioprocess Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Bioresour Bioprocess Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Alemania