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A kinase fusion protein from Aegilops longissima confers resistance to wheat powdery mildew.
He, Huagang; Chen, Zhaozhao; Fan, Renchun; Zhang, Jie; Zhu, Shanying; Wang, Jiale; Zhang, Qianyuan; Gao, Anli; Gong, Shuangjun; Zhang, Lu; Li, Yanan; Zhao, Yitong; Krattinger, Simon G; Shen, Qian-Hua; Li, Hongjie; Wang, Yajun.
Afiliación
  • He H; School of Life Sciences, Jiangsu University, Zhenjiang, China. hghe@ujs.edu.cn.
  • Chen Z; School of Life Sciences, Jiangsu University, Zhenjiang, China.
  • Fan R; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Zhang J; Institute of Biotechnology and Nuclear Technology Research, Sichuan Academy of Agricultural Sciences, Chengdu, China.
  • Zhu S; School of Environment and Safety Engineering, Jiangsu University, Zhenjiang, China.
  • Wang J; School of Life Sciences, Jiangsu University, Zhenjiang, China.
  • Zhang Q; School of Life Sciences, Jiangsu University, Zhenjiang, China.
  • Gao A; School of Life Sciences, Henan University, Kaifeng, China.
  • Gong S; Institute of Plant Protection and Soil Science, Hubei Academy of Agricultural Sciences, Wuhan, China.
  • Zhang L; Key Laboratory of Plant Design, National Key Laboratory of Plant Molecular Genetics, Center for Excellence in Molecular Plant Sciences, Institute of Plant Physiology and Ecology, Chinese Academy of Sciences, Shanghai, China.
  • Li Y; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Zhao Y; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Krattinger SG; Plant Science Program, Biological and Environmental Science & Engineering Division (BESE), King Abdullah University of Science and Technology (KAUST), Thuwal, Saudi Arabia.
  • Shen QH; State Key Laboratory of Plant Cell and Chromosome Engineering, Institute of Genetics and Developmental Biology, The Innovative Academy of Seed Design, Chinese Academy of Sciences, Beijing, China.
  • Li H; Institute of Biotechnology, Xianghu Laboratory, Hangzhou, China.
  • Wang Y; National Engineering Laboratory for Crop Molecular Breeding, Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing, China.
Nat Commun ; 15(1): 6512, 2024 Aug 02.
Article en En | MEDLINE | ID: mdl-39095395
ABSTRACT
Many disease resistance genes have been introgressed into wheat from its wild relatives. However, reduced recombination within the introgressed segments hinders the cloning of the introgressed genes. Here, we have cloned the powdery mildew resistance gene Pm13, which is introgressed into wheat from Aegilops longissima, using a method that combines physical mapping with radiation-induced chromosomal aberrations and transcriptome sequencing analysis of ethyl methanesulfonate (EMS)-induced loss-of-function mutants. Pm13 encodes a kinase fusion protein, designated MLKL-K, with an N-terminal domain of mixed lineage kinase domain-like protein (MLKL_NTD domain) and a C-terminal serine/threonine kinase domain bridged by a brace. The resistance function of Pm13 is validated through transient and stable transgenic complementation assays. Transient over-expression analyses in Nicotiana benthamiana leaves and wheat protoplasts reveal that the fragment Brace-Kinase122-476 of MLKL-K is capable of inducing cell death, which is dependent on a functional kinase domain and the three α-helices in the brace region close to the N-terminus of the kinase domain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Proteínas de Plantas / Ascomicetos / Triticum / Resistencia a la Enfermedad / Aegilops Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Enfermedades de las Plantas / Proteínas de Plantas / Ascomicetos / Triticum / Resistencia a la Enfermedad / Aegilops Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Reino Unido