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Expression of C/EBP and Kr-h1 transcription factors under immune stimulation in the noble crayfish.
Bostjancic, Ljudevit Luka; Dragicevic, Paula; Bonassin, Lena; Francesconi, Caterina; Tarandek, Anita; Schardt, Leonie; Rutz, Christelle; Hudina, Sandra; Schwenk, Klaus; Lecompte, Odile; Theissinger, Kathrin.
Afiliación
  • Bostjancic LL; Institute of Insect Biotechnology, Justus-Liebig University, Heinrich-Buff-Ring 26, 35392 Gießen, Germany; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325 Frankfurt, Germany; Department of Computer Science, ICube, UMR 7357, University of Strasbourg, CNRS
  • Dragicevic P; Depatment of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia.
  • Bonassin L; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325 Frankfurt, Germany; Department of Computer Science, ICube, UMR 7357, University of Strasbourg, CNRS, Centre de Recherche en Biomédecine de Strasbourg, Rue Eugène Boeckel 1, 67000 Strasbourg, France; iES - I
  • Francesconi C; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325 Frankfurt, Germany; iES - Institute for Environmental Sciences, RPTU Kaiserslautern-Landau, Fortstraße 7, 76829 Landau, Germany.
  • Tarandek A; Depatment of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia.
  • Schardt L; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325 Frankfurt, Germany.
  • Rutz C; Department of Computer Science, ICube, UMR 7357, University of Strasbourg, CNRS, Centre de Recherche en Biomédecine de Strasbourg, Rue Eugène Boeckel 1, 67000 Strasbourg, France.
  • Hudina S; Depatment of Biology, Faculty of Science, University of Zagreb, Horvatovac 102a, 10000 Zagreb, Croatia.
  • Schwenk K; iES - Institute for Environmental Sciences, RPTU Kaiserslautern-Landau, Fortstraße 7, 76829 Landau, Germany.
  • Lecompte O; Department of Computer Science, ICube, UMR 7357, University of Strasbourg, CNRS, Centre de Recherche en Biomédecine de Strasbourg, Rue Eugène Boeckel 1, 67000 Strasbourg, France.
  • Theissinger K; Institute of Insect Biotechnology, Justus-Liebig University, Heinrich-Buff-Ring 26, 35392 Gießen, Germany; LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Senckenberganlage 25, 60325 Frankfurt, Germany.
Gene ; 929: 148813, 2024 Dec 15.
Article en En | MEDLINE | ID: mdl-39094714
ABSTRACT
Transcription factors (TFs) have an important role in the regulation of the gene expression network. The role of TFs in the immune response of freshwater crayfish is poorly understood, but leveraging the regulatory mechanisms of immune response could augment the resistance against the invasive oomycete pathogen, Aphanomyces astaci. Previous studies indicated that the TFs CCAAT/enhancer-binding protein (C/EBP) and putative Krüppel homolog-1 protein (Kr-h1) might play a role in immune and stress response of the noble crayfish (Astacus astacus). Here, we aimed to further characterise these two gene products to gain a better understanding of their evolutionary origin, domain organisation and expression patterns across different crayfish tissues. Furthermore, we conducted an immune stimulation experiment to observe the potential changes in the gene expression of C/EBP and Kr-h1 under immune challenge in different crayfish tissues. Our results showed that both C/EBP and Kr-h1 are closely related to other C/EBPs and Kr-h1s in Malacostraca. Gene expression analysis revealed that both TFs are present in all analysed tissues, with higher expression of C/EBP in the gills and Kr-h1 in the abdominal muscle. Immune stimulation with laminarin (mimicking ß-1-3-glucan in the oomycete cell wall) showed an activation of the crayfish immune system, with an overall increase in the total haemocyte count (THC) compared to untreated control and crayfish buffered saline (CBS) treatment. On the gene expression level, an up-regulation of the C/EBP gene was detected in the laminarin treated group in hepatopancreas and heart, while no changes were observed for the Kr-h1 gene. Our results indicate an early change in C/EBP expression in multiple tissues during immune stimulation and suggest its involvement in the immune response of the noble crayfish.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Astacoidea Límite: Animals Idioma: En Revista: Gene Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Astacoidea Límite: Animals Idioma: En Revista: Gene Año: 2024 Tipo del documento: Article Pais de publicación: Países Bajos