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Protocol for evaluating S-acylated protein membrane affinity using protein-lipid conjugates.
Xie, Chunyang; Luo, Dong; Liu, Lu; Huang, Xue; Zhang, Shao-Lin; Yang, Aimin.
Afiliación
  • Xie C; School of Life Sciences, Chongqing University, Chongqing 401331, China.
  • Luo D; School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China.
  • Liu L; School of Life Sciences, Chongqing University, Chongqing 401331, China.
  • Huang X; School of Life Sciences, Chongqing University, Chongqing 401331, China.
  • Zhang SL; School of Pharmaceutical Sciences, Chongqing University, Chongqing 401331, China. Electronic address: zhangsl@cqu.edu.cn.
  • Yang A; School of Life Sciences, Chongqing University, Chongqing 401331, China. Electronic address: aimin.yang@cqu.edu.cn.
STAR Protoc ; 5(3): 103182, 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-39093703
ABSTRACT
S-acylation of proteins allows their association with membranes. Here, we present a protocol for establishing a platform for membrane affinity evaluation of S-acylated proteins in vitro. We describe steps for preparing lipid-maleimide compounds, mCherry-p62 recombinant proteins, and total cellular membranes. We then detail procedures for synthesizing protein-lipid conjugates using lipid-maleimide compounds and recombinant proteins and evaluating the membrane affinity of protein-lipid conjugates. For complete details on the use and execution of this protocol, please refer to Huang Xue et al.1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Membrana Celular Límite: Animals / Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Membrana Celular Límite: Animals / Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos