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Complete de novo assembly of Wolbachia endosymbiont of Drosophila willistoni using long-read genome sequencing.
Jacobs, Jodie; Nakamoto, Anne; Mastoras, Mira; Loucks, Hailey; Mirchandani, Cade; Karim, Lily; Penunuri, Gabriel; Wanket, Ciara; Russell, Shelbi L.
Afiliación
  • Jacobs J; Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.
  • Nakamoto A; Genomics Institute, University of California, Santa Cruz, CA, USA.
  • Mastoras M; Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.
  • Loucks H; Genomics Institute, University of California, Santa Cruz, CA, USA.
  • Mirchandani C; Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.
  • Karim L; Genomics Institute, University of California, Santa Cruz, CA, USA.
  • Penunuri G; Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.
  • Wanket C; Genomics Institute, University of California, Santa Cruz, CA, USA.
  • Russell SL; Department of Biomolecular Engineering, University of California, Santa Cruz, CA, USA.
Sci Rep ; 14(1): 17770, 2024 08 01.
Article en En | MEDLINE | ID: mdl-39090271
ABSTRACT
Wolbachia is an obligate intracellular α-proteobacterium, which commonly infects arthropods and filarial nematodes. Different strains of Wolbachia are capable of a wide range of regulatory manipulations in their diverse hosts, including the modulation of host cellular differentiation to influence host reproduction. The genetic basis for the majority of these phenotypes is unknown. The wWil strain from the neotropical fruit fly, Drosophila willistoni, exhibits a remarkably high affinity for host germline-derived cells relative to the somatic cells. This trait could be leveraged for understanding how Wolbachia influences the host germline and for controlling host populations in the field. To further the use of this strain in biological and biomedical research, we sequenced the genome of the wWil strain isolated from host cell culture cells. Here, we present the first high quality Nanopore assembly of wWil, the Wolbachia endosymbiont of D. willistoni. Our assembly resulted in a circular genome of 1.27 Mb with a BUSCO completeness score of 99.7%. Consistent with other insect-associated Wolbachia strains, comparative genomic analysis revealed that wWil has a highly mosaic genome relative to the closely related wMel and wAu strains from Drosophila melanogaster and Drosophila simulans, respectively.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simbiosis / Genoma Bacteriano / Wolbachia / Drosophila Límite: Animals Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Simbiosis / Genoma Bacteriano / Wolbachia / Drosophila Límite: Animals Idioma: En Revista: Sci Rep Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido