The effect of miR1555p on longterm memory of sleepdeprived mice through the BDNF/NFκB pathway.
Acta Neurobiol Exp (Wars)
; 84(2): 203-217, 2024 Jun 28.
Article
en En
| MEDLINE
| ID: mdl-39087835
ABSTRACT
Sleep deprivation (SD) is a prevalent sleep issue in modern society that significantly impairs neurological function and quality of life in affected individuals. This study seeks to investigate the involvement of the miR1555p/BDNF axis in SD mice, aiming to establish a theoretical foundation for potential treatment strategies. Male C57BL/6 mice were utilized in the construction of a SD model using the flower pot technique. HT22 cells were selected for cellular experiments. The Morris water maze was employed to assess the learning and memory capabilities of the mice. HE staining was utilized to observe pathological changes in hippocampal tissue. Levels of IL1ß, IL6, and TNFα were analyzed using ELISA. The expression level of miR1555p was quantified via RTqPCR. The binding between miR1555p and brainderived neurotrophic factor (BDNF) was confirmed through a dualluciferase reporter assay. Apoptosis of hippocampal neurons was assessed using TUNEL. Western blot analysis was conducted to evaluate the expression levels of BDNF, p65, and pp65. The Morris water maze test revealed that the mice exhibited prolonged escape latency, decreased swimming velocity, and reduced time spent in the target platform quadrant, which are indicative of a successful construction of the SD model. The observed cognitive deficits in the mice were associated with SDinduced damage to the hippocampal tissue, leading to increased levels of miR1555p and decreased levels of BDNF. miR1555p was found to directly bind to BDNF, thereby suppressing its mRNA and protein expression. The upregulation of BDNF effectively mitigated hippocampal damage by attenuating cell apoptosis and reducing inflammation levels in SD mice. Additionally, the BDNF/NFκB pathway was found to be suppressed in SD mice through the downregulation of miR1555p. Therefore, the silencing of miR1555p inhibited the activation of the NFκB pathway by upregulating BDNF, which improved longterm memory and reduced neuronal damage in SD mice.
Texto completo:
1
Colección:
01-internacional
Base de datos:
MEDLINE
Asunto principal:
Privación de Sueño
/
Transducción de Señal
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FN-kappa B
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Factor Neurotrófico Derivado del Encéfalo
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MicroARNs
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Memoria a Largo Plazo
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Hipocampo
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Ratones Endogámicos C57BL
Límite:
Animals
Idioma:
En
Revista:
Acta Neurobiol Exp (Wars)
Año:
2024
Tipo del documento:
Article
País de afiliación:
China
Pais de publicación:
Polonia