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Reduced Abundance of Phocaeicola in Mucosa-associated Microbiota Is Associated with Distal Colorectal Cancer Metastases Possibly through an Altered Local Immune Environment.
Ota, Gaku; Inoue, Ryo; Saito, Akira; Kono, Yoshihiko; Kitayama, Joji; Sata, Naohiro; Horie, Hisanaga.
Afiliación
  • Ota G; Department of Surgery, Jichi Medical University, Shimotsuke, Japan.
  • Inoue R; Laboratory of Animal Science, Department of Applied Biological Sciences, Faculty of Agriculture, Setsunan University, Hirakata, Japan.
  • Saito A; Department of Surgery, Jichi Medical University, Shimotsuke, Japan.
  • Kono Y; Department of Surgery, Jichi Medical University, Shimotsuke, Japan.
  • Kitayama J; Department of Surgery, Jichi Medical University, Shimotsuke, Japan.
  • Sata N; Clinical Research Center, Division of Translational Research, Jichi Medical University, Shimotsuke, Japan.
  • Horie H; Department of Surgery, Jichi Medical University, Shimotsuke, Japan.
J Anus Rectum Colon ; 8(3): 235-245, 2024.
Article en En | MEDLINE | ID: mdl-39086872
ABSTRACT

Objectives:

The aim of this study was to identify the microbiota whose decrease in tumor area was associated with the metastatic process of distal colorectal cancer (CRC).

Methods:

Twenty-eight consecutive patients with distal CRC undergoing surgical resection in our hospital were enrolled. Microbiota in 28 specimens from surgically resected colorectal cancers were analyzed using 16S ribosomal ribonucleic acid gene amplicon sequencing and the relative abundance (RA) of microbiota was evaluated. The densities of tumor-infiltrating lymphocytes (TIL) and tumor associated macrophages (TAM) in the colorectal cancers were immunohistochemically evaluated.

Results:

Phocaeicola was the most abundant microbiota in normal mucosa. The RA of Phocaeicola in tumor tissues tended to be lower than that in normal mucosa although the difference was not significant (p=0.0732). The RA of Phocaeicola at tumor sites did not correlate either with depth of tumor invasion (pT-stage) or tumor size, however they were significantly reduced in patients with nodal metastases (p<0.05) and those with distant metastases (p<0.001). The RA of Phocaeicola at tumor sites showed positive correlation with the densities of CD3(+) or CD8(+) TIL. Since P. vulgatus was the most dominant species (47%) of the Phocaeicola, the RA of P. vulgatus and CRC metastasis and its association with TIL and TAM were also investigated. P. vulgatus showed a similar trend to genus Phocaeicola but was not statistically significant.

Conclusions:

A relative reduction of Phocaeicola attenuates the local anti-tumor immune response in distal CRC, which may facilitate metastatic spread.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Anus Rectum Colon Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Anus Rectum Colon Año: 2024 Tipo del documento: Article País de afiliación: Japón Pais de publicación: Japón