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Methylome-wide analysis in systemic microbial-induced experimental periodontal disease in mice with different susceptibility.
Hernandez Martinez, Cristhiam de Jesus; Glessner, Joseph; Finoti, Livia Sertori; Silva, Pedro Felix; Messora, Michel; Coletta, Ricardo Della; Hakonarson, Hakon; Palioto, Daniela Bazan.
Afiliación
  • Hernandez Martinez CJ; Department of Oral & Maxillofacial Surgery and Periodontology, Ribeirão Preto Dental School, University of São Paulo - USP, Ribeirão Preto, São Paulo, Brazil.
  • Glessner J; The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, United States.
  • Finoti LS; The Center for Applied Genomics, Children's Hospital of Philadelphia, Philadelphia, PA, United States.
  • Silva PF; Department of Pediatrics, The Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, United States.
  • Messora M; Division of Human Genetics, Children's Hospital of Philadelphia, Philadelphia, PA, United States.
  • Coletta RD; Laboratory of Rebecca Ahrens-Nicklas,Children's Hospital of Philadelphia, Philadelphia, PA, United States.
  • Hakonarson H; Department of Oral & Maxillofacial Surgery and Periodontology, Ribeirão Preto Dental School, University of São Paulo - USP, Ribeirão Preto, São Paulo, Brazil.
  • Palioto DB; Department of Oral & Maxillofacial Surgery and Periodontology, Ribeirão Preto Dental School, University of São Paulo - USP, Ribeirão Preto, São Paulo, Brazil.
Front Cell Infect Microbiol ; 14: 1369226, 2024.
Article en En | MEDLINE | ID: mdl-39086605
ABSTRACT

Objective:

The study delved into the epigenetic factors associated with periodontal disease in two lineages of mice, namely C57bl/6 and Balb/c. Its primary objective was to elucidate alterations in the methylome of mice with distinct genetic backgrounds following systemic microbial challenge, employing high-throughput DNA methylation analysis as the investigative tool.

Methods:

Porphyromonas gingivalis (Pg)was orally administered to induce periodontitis in both Balb/c and C57bl/6 lineage. After euthanasia, genomic DNA from both maxilla and blood were subjected to bisulfite conversion, PCR amplification and genome-wide DNA methylation analysis using the Ovation RRBS Methyl-Seq System coupled with the Illumina Infinium Mouse Methylation BeadChip.

Results:

Of particular significance was the distinct methylation profile observed within the Pg-induced group of the Balb/c lineage, contrasting with both the control and Pg-induced groups of the C57bl/6 lineage. Utilizing rigorous filtering criteria, we successfully identified a substantial number of differentially methylated regions (DMRs) across various tissues and comparison groups, shedding light on the prevailing hypermethylation in non-induced cohorts and hypomethylation in induced groups. The comparison between blood and maxilla samples underscored the unique methylation patterns specific to the jaw tissue. Our comprehensive methylome analysis further unveiled statistically significant disparities, particularly within promoter regions, in several comparison groups.

Conclusion:

The differential DNA methylation patterns observed between C57bl/6 and Balb/c mouse lines suggest that epigenetic factors contribute to the variations in disease susceptibility. The identified differentially methylated regions associated with immune regulation and inflammatory response provide potential targets for further investigation. These findings emphasize the importance of considering epigenetic mechanisms in the development and progression of periodontitis.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Porphyromonas gingivalis / Metilación de ADN / Modelos Animales de Enfermedad / Ratones Endogámicos BALB C / Ratones Endogámicos C57BL Límite: Animals Idioma: En Revista: Front Cell Infect Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Porphyromonas gingivalis / Metilación de ADN / Modelos Animales de Enfermedad / Ratones Endogámicos BALB C / Ratones Endogámicos C57BL Límite: Animals Idioma: En Revista: Front Cell Infect Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Suiza