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Expression patterns of immune checkpoint proteins and Plasmodium falciparum-induced cytokines in chronic hepatitis B virus-infected and uninfected individuals: A cross-sectional study.
Segbefia, Selorm P; Asandem, Diana A; Pobee, Abigail; Asare, Bright; Prah, Ahu Diana; Baba-Adam, Rawdat; Amponsah, Jones Amo; Kyei-Baafour, Eric; van der Puije, William; Osei, Frank; Teye-Adjei, Doreen; Agyemang, Seth; Brenko, Theophilus; Bentum-Ennin, Lutterodt; Tetteh, John K A; Bonney, Kofi J H; Sakyi, Samuel Asamoah; Amoah, Linda E; Kusi, Kwadwo A.
Afiliación
  • Segbefia SP; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Asandem DA; Department of Molecular Medicine, School of Medicine and Dentistry College of Health Sciences, KNUST Kumasi Ghana.
  • Pobee A; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana.
  • Asare B; Department of Virology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Prah AD; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Baba-Adam R; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Amponsah JA; Department of Animal Biology and Conservation Science, College of Basic and Applied Sciences University of Ghana Accra Ghana.
  • Kyei-Baafour E; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana.
  • van der Puije W; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Osei F; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana.
  • Teye-Adjei D; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Agyemang S; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Brenko T; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Bentum-Ennin L; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Tetteh JKA; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Bonney KJH; Department of Biochemistry, Cell and Molecular Biology, West African Centre for Cell Biology of Infectious Pathogens, College of Basic and Applied Sciences University of Ghana Accra Ghana.
  • Sakyi SA; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Amoah LE; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
  • Kusi KA; Department of Immunology, NMIMR, College of Health Sciences University of Ghana Accra Ghana.
Health Sci Rep ; 7(8): e2280, 2024 Aug.
Article en En | MEDLINE | ID: mdl-39086506
ABSTRACT
Background and

Aim:

Chronic hepatitis B virus (CHB) infection remains a major public health problem. The American Association for the Study of Liver Diseases (AASLD) 2018 Hepatitis B Guidelines provide that CHB individuals not requiring antiviral therapy yet are monitored to determine the need for antiviral therapy in the future; however, these tests do not include measurement of cytokines and immune cell characterization. This case-control study compared the cytokine and immune checkpoint protein expression profiles between CHB individuals not yet on antiviral treatment and hepatitis B virus (HBV)-negative individuals.

Methods:

CD4 and CD8 T cells from CHB and HBV-negative individuals were characterized for immune checkpoint proteins programmed cell death-1 (PD1), T cell Immunoglobulin domain and mucin domain-containing protein 3 (TIM-3), and cytotoxic T lymphocyte-associated antigen 4 (CTLA-4) (CD152), and a memory marker CXCR3 (CD183) using flow cytometry. Malaria-induced cytokine expression levels were determined by stimulating their blood cells with Plasmodium falciparum 3D7 strain antigens (CSP, AMA-1, and TRAP) in whole blood assays, and cytokine levels were measured using a 13-plex Luminex kit.

Results:

HBV-negative and CHB individuals had comparable levels of CD4+ and CD8+ T cells. However, a proportion of the CD4+ and CD8+ populations from both groups, which were CXCR3+, expressed PD-1 and CD152. The ability to produce cytokines in response to malaria antigen stimulation was not significantly different between the groups.

Conclusion:

These findings support excluding CHB individuals from antiviral therapy at this stage of infection. However, CHB individuals require regular monitoring to determine the need for later antiviral treatment.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Health Sci Rep Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Health Sci Rep Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos