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GABA-gated chloride channel mutation (Rdl) induces cholinergic physiological compensation resulting in cross resistance in Drosophila melanogaster.
Xie, Na; Bickley, Brandon A; Gross, Aaron D.
Afiliación
  • Xie N; Virginia Polytechnic Institute and State University, Department of Entomology, Molecular Physiology and Toxicology Laboratory Blacksburg, VA 24061, USA.
  • Bickley BA; Virginia Polytechnic Institute and State University, Department of Entomology, Molecular Physiology and Toxicology Laboratory Blacksburg, VA 24061, USA.
  • Gross AD; Virginia Polytechnic Institute and State University, Department of Entomology, Molecular Physiology and Toxicology Laboratory Blacksburg, VA 24061, USA; School of Neuroscience, Fralin Life Science Institute, Virginia Tech Center for Drug Discovery, Center for Emerging Zoonotic and Arthropod-borne Diseases, Virginia Tech, Blacksburg, VA 24061, USA. Electronic address: adgross@vt.edu.
Pestic Biochem Physiol ; 203: 105972, 2024 Aug.
Article en En | MEDLINE | ID: mdl-39084765
ABSTRACT
The Drosophila melanogaster MD-RR strain contains an Rdl mutation (A301S) resulting in resistance to several insecticide classes viz. phenyl pyrazoles (e.g., fipronil), cyclodienes (e.g., dieldrin), and chlorinated aliphatic hydrocarbons (e.g., lindane). Fitness costs are commonly observed with resistant insect populations as side effects of the genetic change conferring the resistant phenotype. Because of fitness costs, reversion from the resistant to susceptible genotype and phenotype is common. However, the Rdl genotype in D. melanogaster appears to allow the flies to maintain the resistant genotype/phenotype without selective pressure and with minimal fitness costs. We provide evidence that compensation for the Rdl mutation influences the cholinergic system, where an increase in acetylcholinesterase gene expression and enzyme activity results in neurophysiological changes and cross resistance to a carbamate insecticide (propoxur oral resistance ratio (RR) of 63) and an organophosphate insecticide (dichlorvos oral RR of 7). Such cross resistance was not previously reported with the initial collection and testing of this strain. In addition to acetylcholinesterase, the Rdl mutation influences the expression of the muscarinic acetylcholine receptor subtype-B, resulting in resistance to non-selective muscarinic compounds (pilocarpine and atropine). Collectively, these results indicate that the Rdl mutation (A301S) at GABA-gated ionophore complex influences the physiology of the cholinergic system, leading to resistance to established insecticide classes. Additionally, this mutation may impact the effectiveness of insecticides targeting novel sites, like muscarinic receptors.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetilcolinesterasa / Resistencia a los Insecticidas / Canales de Cloruro / Receptores de GABA-A / Proteínas de Drosophila / Drosophila melanogaster Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Acetilcolinesterasa / Resistencia a los Insecticidas / Canales de Cloruro / Receptores de GABA-A / Proteínas de Drosophila / Drosophila melanogaster Límite: Animals Idioma: En Revista: Pestic Biochem Physiol Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos