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Dual-enhanced enzyme cascade hybrid hydrogel for the construction of optical biosensor.
Zou, Ruiqi; Li, Hongxia; Shi, Junxiao; Sun, Chunyan; Lu, Geyu; Yan, Xu.
Afiliación
  • Zou R; Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China.
  • Li H; Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China. Electronic address: hxiali@jlu.edu.cn.
  • Shi J; Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China.
  • Sun C; Department of Food Quality and Safety, College of Food Science and Engineering, Jilin University, Changchun, 130062, PR China.
  • Lu G; Key Laboratory of Advanced Gas Sensors, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, PR China.
  • Yan X; Key Laboratory of Advanced Gas Sensors, College of Electronic Science and Engineering, Jilin University, Changchun, 130012, PR China. Electronic address: yanx@jlu.edu.cn.
Biosens Bioelectron ; 263: 116613, 2024 Nov 01.
Article en En | MEDLINE | ID: mdl-39084044
ABSTRACT
The biomimetic enzyme cascade system plays a key role in biosensing as a sophisticated signal transduction and amplification strategy. However, constructing a regulated enzyme cascade sensing system remains challenging due to the mismatch of multiple enzyme activities and poor stability. Herein, we design an efficient dual-enhanced enzyme cascade hybrid system (UFD-DEC) containing DNA-controlled nanozymes (Fe-cdDNA) and enzyme (urease) via combining the electrostatic contact effect with the hydrogel-directed confinement effect. Precise modulation of Fe-cdDNA nanozyme by DNA offers a means to control its catalytic efficiency. This regulated UFD-DEC system accelerates the reaction rate and provides remarkable stability compared with the free enzyme system. Benefiting from the plasticity properties of hydrogels, a "lab-in-a-tube" platform was constructed by encapsulating UFD-DEC in a microcentrifuge tube. Such a UFD-DEC-based hydrogel tube exhibits sufficient adaptability to profile urea when used in conjunction with a smartphone-assisted image processing algorithm, which on-site delivers urea information with a detection limit of 0.12 mmol L-1. This customizable and inexpensive miniaturized biosensor platform for monitoring urea may facilitate point-of-care testing applications.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ureasa / Técnicas Biosensibles / Hidrogeles / Límite de Detección Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
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XML
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ureasa / Técnicas Biosensibles / Hidrogeles / Límite de Detección Idioma: En Revista: Biosens Bioelectron Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido