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A multi-nutrient array protocol to study disease-diet interactions in Drosophila melanogaster.
Lin, Jiayi; Martelli, Felipe; Mele, Sarah; Quig, Annelise; Johnson, Travis K; Piper, Matthew D W.
Afiliación
  • Lin J; School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia.
  • Martelli F; School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia.
  • Mele S; School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia.
  • Quig A; School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia.
  • Johnson TK; Department of Biochemistry and Chemistry and La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC 3086, Australia. Electronic address: t.johnson@latrobe.edu.au.
  • Piper MDW; School of Biological Sciences, Monash University, Clayton, VIC 3800, Australia. Electronic address: matthew.piper@monash.edu.
STAR Protoc ; 5(3): 103225, 2024 Jul 30.
Article en En | MEDLINE | ID: mdl-39083384
ABSTRACT
The availability of a defined fully synthetic diet for the fruit fly Drosophila melanogaster allows for complete and precise manipulation of its nutritional environment. Here, we present a protocol for performing large-scale multivariate nutrient analysis via the traditional diet preparation approach, or by adding nutrient solutions to a baseline medium. We detail procedures from sample collection to data analysis. This protocol has applications for the study of nutrition-life trait interactions and nutrigenomics, to reveal interactions between genotype and diet composition. For complete details on the use and execution of this protocol, please refer to Martelli et al.1 and Martelli et al.2.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: Australia Pais de publicación: Estados Unidos