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Enteroaggregative Escherichia coli (EAEC) isolates obtained from non-diarrheic children carry virulence factor-encoding genes from Extraintestinal Pathogenic E. Coli (ExPEC).
Luiz, Bruna M; Cergole-Novella, Maria C; Dantas, Stéfani T A; de Lira, Daiany R P; de Souza, Guilherme F R; Fernandes, Iranildo do A; Orsi, Henrique; Solveira, Guilherme; Rall, Vera L M; Dos Santos, Luís F; Hernandes, Rodrigo T.
Afiliación
  • Luiz BM; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • Cergole-Novella MC; Laboratório Regional de Santo André, Instituto Adolfo Lutz, Santo André, SP, Brasil.
  • Dantas STA; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • de Lira DRP; Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, Pirassununga, SP, Brasil.
  • de Souza GFR; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • Fernandes IDA; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • Orsi H; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • Solveira G; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • Rall VLM; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • Dos Santos LF; Instituto de Biociências, Universidade Estadual Paulista (UNESP), Botucatu, SP, Brasil.
  • Hernandes RT; Centro de Bacteriologia, Instituto Adolfo Lutz, São Paulo, SP, Brasil.
Braz J Microbiol ; 2024 Jul 31.
Article en En | MEDLINE | ID: mdl-39083223
ABSTRACT
Enteroaggregative E. coli (EAEC) is one of the most frequent pathogens isolated from diarrheal patients as well as from healthy individuals in Brazil and has recently also been implicated as an extraintestinal pathogenic E. coli (ExPEC) associated with bloodstream and urinary tract infections. In this study, 37 EAEC isolates, obtained from fecal samples of non-diarrheic children, were molecularly and phenotypically characterized to access the pathogenic features of these isolates. The EAEC isolates were assigned into the phylogroups A (54.1%), D (29.7%), B1 (13.5%) and B2 (2.7%); and harbored genes responsible for encoding the major pilin subunit of the aggregative adherence fimbriae (AAFs) or aggregate-forming pili (AFP) adhesins as follows aggA (24.3%), agg3A (5.4%), agg4A (27.0%), agg5A (32.4%) and afpA (10.8%). The most frequent OH serotypes were O15H2 (8.1%), O38H25 (5.4%) and O86H2 (5.4%). Twenty-one isolates (56.8%) produce the aggregative adherence (AA) pattern on HeLa cells, and biofilm formation was more efficient among EAEC isolates harboring the aggA and agg5A genes. PFGE analysis showed that 31 (83.8%) of the isolates were classified into 10 distinct clusters, which reinforces the high diversity found among the isolates studied. Of note, 40.5% (15/37) of the EAEC isolates have a genetic profile compatible with E. coli isolates with intrinsic potential to cause extraintestinal infections in healthy individuals, and therefore, classified as EAEC/ExPEC hybrids. In conclusion, we showed the presence of EAEC/ExPEC hybrids in the intestinal microbiota of non-diarrheic children, possibly representing the source of some endogenous extraintestinal infections.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Braz J Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Braz J Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Brasil