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Co-transcriptional production of programmable RNA condensates and synthetic organelles.
Fabrini, Giacomo; Farag, Nada; Nuccio, Sabrina Pia; Li, Shiyi; Stewart, Jaimie Marie; Tang, Anli A; McCoy, Reece; Owens, Róisín M; Rothemund, Paul W K; Franco, Elisa; Di Antonio, Marco; Di Michele, Lorenzo.
Afiliación
  • Fabrini G; Department of Chemistry, Imperial College London, London, UK.
  • Farag N; fabriCELL, Imperial College London, London, UK.
  • Nuccio SP; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
  • Li S; The Francis Crick Institute, London, UK.
  • Stewart JM; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
  • Tang AA; Department of Chemistry, Imperial College London, London, UK.
  • McCoy R; Department of Bioengineering, University of California at Los Angeles, Los Angeles, CA, USA.
  • Owens RM; Department of Computing and Mathematical Sciences, California Institute of Technology, Pasadena, CA, USA.
  • Rothemund PWK; Department of Mechanical and Aerospace Engineering, University of California at Los Angeles, Los Angeles, CA, USA.
  • Franco E; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
  • Di Antonio M; Department of Chemical Engineering and Biotechnology, University of Cambridge, Cambridge, UK.
  • Di Michele L; Department of Computing and Mathematical Sciences, California Institute of Technology, Pasadena, CA, USA.
Nat Nanotechnol ; 2024 Jul 30.
Article en En | MEDLINE | ID: mdl-39080489
ABSTRACT
Condensation of RNA and proteins is central to cellular functions, and the ability to program it would be valuable in synthetic biology and synthetic cell science. Here we introduce a modular platform for engineering synthetic RNA condensates from tailor-made, branched RNA nanostructures that fold and assemble co-transcriptionally. Up to three orthogonal condensates can form simultaneously and selectively accumulate fluorophores through embedded fluorescent light-up aptamers. The RNA condensates can be expressed within synthetic cells to produce membrane-less organelles with a controlled number and relative size, and showing the ability to capture proteins using selective protein-binding aptamers. The affinity between otherwise orthogonal nanostructures can be modulated by introducing dedicated linker constructs, enabling the production of bi-phasic RNA condensates with a prescribed degree of interphase mixing and diverse morphologies. The in situ expression of programmable RNA condensates could underpin the spatial organization of functionalities in both biological and synthetic cells.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nat Nanotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Nat Nanotechnol Año: 2024 Tipo del documento: Article Pais de publicación: Reino Unido