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Genotype classification and pathogenicity of infectious bursal disease virus circulating in vaccinated broiler chicken farms.
Mosad, Samah M; Elsayed, Mona M; Hammad, Enas M; Hendam, Basma M; Ali, Hanaa S; Eladl, Abdelfattah H; Saif, Mohamed A.
Afiliación
  • Mosad SM; Department of Virology, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
  • Elsayed MM; Department of Hygiene and Zoonoses, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
  • Hammad EM; Department of Poultry Diseases, Agricultural Research Center, Animal Health Research Institute, Mansoura Branch, Giza, Egypt.
  • Hendam BM; Department of Animal Wealth Development, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt.
  • Ali HS; Department of Pathology, Agricultural Research Center, Animal Health Research Institute, Mansoura Branch, Giza, Egypt.
  • Eladl AH; Department of Poultry Diseases, Faculty of Veterinary Medicine, Mansoura University, Mansoura, Egypt. abdelfatahpo@yahoo.com.
  • Saif MA; Department of Virology, Reference Laboratory for Veterinary Quality Control On Poultry Production (Gamasa Branch), Agricultural Research Center, Animal Health Research Institute, Giza, Egypt.
Vet Res Commun ; 2024 Jul 30.
Article en En | MEDLINE | ID: mdl-39078474
ABSTRACT
This study investigated the genotype classification and pathogenicity of infectious bursal disease virus (IBDV) circulating in vaccinated broiler chicken farms in Egypt. A total of 150 samples were collected from 30 vaccinated commercial broiler chicken farms and pooled into 30 working samples. IBDV was tested using reverse transcriptase polymerase chain reaction (RT-PCR) amplification of the hypervariable region of the viral protein 2 (hvVP2) and the VP1 gene 5' extremity. Both RT-PCR fragments were sequenced from six samples, and then the obtained nucleotide sequences were analyzed. The IBDV genotypes were identified using nucleotide sequences. Five sequences of the six strains examined were classified as genotype A3B2 for the highly virulent segments A and B (vv-A/vv-B IBDV). Interestingly, this study identified and classified a novel segment-reassortant strain as the A1B2 genotype. Specifically, it involved the segment reassortment of classical virulent segment A (cv-A) with vv-B producing cv-A/vv-B reassortant IBDV. Subsequently, we compared the pathogenicity of reassortant (cv-A/vv-B) IBDV and vvIBDV strains identified in this study. Both strains developed typical IBD clinical signs, postmortem lesions, histopathology, immunohistochemistry, and lesion scores, which were more severe in vvIBDV than reassortant IBDV. In conclusion, this is the first report of the genotype classification based on both genome segments (hvVP2 and VP1) with pathogenicity of IBDV circulating in vaccinated broiler chicken farms and this pathogenicity is more severe in vvIBDV strain than a novel reassortant IBDV strain.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Vet Res Commun Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza