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Coexistence of a novel NDM-1-encoding MDR plasmid and an IMP-4-encoding IncN-IncU hybrid plasmid in a clinical isolate of Citrobacter freundii BC73.
Liu, Na; Tang, Biao; Wang, Hui; Chen, Xiangyang; Wen, Peipei; Wang, Zhaorui; Chen, Xu; Guo, Xiaobing; Gou, Jianjun; Song, Yinsen.
Afiliación
  • Liu N; Translational Medicine Research Center, Zhengzhou People's Hospital, The Fifth Clinical College of Henan University of Chinese Medicine, Zhengzhou, China.
  • Tang B; Key Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.
  • Wang H; Translational Medicine Research Center, Zhengzhou People's Hospital, The Fifth Clinical College of Henan University of Chinese Medicine, Zhengzhou, China.
  • Chen X; Department of Laboratory Medicine, Zhengzhou People's Hospital, The Fifth Clinical College of Henan University of Chinese Medicine, Zhengzhou, China.
  • Wen P; Translational Medicine Research Center, Zhengzhou People's Hospital, The Fifth Clinical College of Henan University of Chinese Medicine, Zhengzhou, China.
  • Wang Z; Translational Medicine Research Center, Zhengzhou People's Hospital, The Fifth Clinical College of Henan University of Chinese Medicine, Zhengzhou, China.
  • Chen X; Translational Medicine Research Center, Zhengzhou People's Hospital, The Fifth Clinical College of Henan University of Chinese Medicine, Zhengzhou, China.
  • Guo X; Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Gou J; Department of Laboratory Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
  • Song Y; Translational Medicine Research Center, Zhengzhou People's Hospital, The Fifth Clinical College of Henan University of Chinese Medicine, Zhengzhou, China.
Front Microbiol ; 15: 1388651, 2024.
Article en En | MEDLINE | ID: mdl-39077736
ABSTRACT

Objectives:

To investigate the genetic characteristics and transmission mechanism of the NDM-1-, IMP-4-, and SHV-12-producing multidrug-resistant (MDR) clinical isolate, Citrobacter freundii BC73.

Methods:

C. freundii BC73 was isolated from a urine specimen of a urological patient diagnosed with bladder cancer at a Chinese teaching hospital. Antimicrobial susceptibility testing was carried out using DL-120E susceptibility cards and DL-96A system. Whole genome sequencing (WGS) of the isolate was performed using the Illumina and Oxford Nanopore platforms to analyze the genetic context of drug resistance genes and plasmid characteristics. The phylogenetic tree was constructed and visualized by KSNP3.0 software and iTOL5.0 online database.

Results:

C. freundii isolate BC73 co-carrying bla NDM-1, bla IMP-4 and bla SHV-12 were multidrug-resistant. bla NDM-1 and bla IMP-4 were located on a novel IncFIB-like plasmid, pCFBC1, and an IncN-IncU hybrid plasmid, pCFBC2, respectively. The transferability of bla NDM-1 and bla IMP-4 from C. freundii BC73 to E. coli J53 was successfully demonstrated. The genetic context of the bla NDM-1 and bla IMP-4 genes were ISCR27-groEL-∆groES-cutA-dsbD-trpF-ble MBL-bla NDM-1-∆ISAba125-IS3000 and intI1-bla IMP-4-Kl.pn.13-mobC-IS6100, respectively. Additionally, two extensive transposition units (MGE1 in pCFBC1, MGE2 in pCFBC2) were identified and numerous antimicrobial resistance genes were discovered on it.

Conclusion:

To our knowledge, our study represents the first characterization of a ST22 C. freundii isolate co-harboring bla NDM-1, bla IMP-4, and bla SHV-12, obtained from a urine sample. The dissemination of this MDR isolate should be of close concern in future clinical surveillance.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza