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Wastewater-based epidemiological investigation of SARS-CoV-2 in Porto Alegre, Southern Brazil.
Aschidamini Prandi, Bruno; Mangini, Arthur Tonietto; Santiago Neto, Waldemir; Jarenkow, André; Violet-Lozano, Lina; Campos, Aline Alves Scarpellini; Colares, Evandro Ricardo da Costa; Buzzetto, Paula Regina de Oliveira; Azambuja, Camila Bernardes; Trombin, Lisiane Correa de Barros; Raugust, Margot de Souza; Lorenzini, Rafaela; Larre, Alberto da Silva; Rigotto, Caroline; Campos, Fabrício Souza; Franco, Ana Cláudia.
Afiliación
  • Aschidamini Prandi B; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Brazil.
  • Mangini AT; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Brazil.
  • Santiago Neto W; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Brazil.
  • Jarenkow A; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, Rio Grande do Sul, 90119-900, Brazil.
  • Violet-Lozano L; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Brazil.
  • Campos AAS; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Brazil.
  • Colares ERDC; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, Rio Grande do Sul, 90119-900, Brazil.
  • Buzzetto PRO; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, Rio Grande do Sul, 90119-900, Brazil.
  • Azambuja CB; Municipal Water and Sewage department, Porto Alegre, Rio Grande do Sul, Brazil.
  • Trombin LCB; Municipal Water and Sewage department, Porto Alegre, Rio Grande do Sul, Brazil.
  • Raugust MS; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, Rio Grande do Sul, 90119-900, Brazil.
  • Lorenzini R; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, Rio Grande do Sul, 90119-900, Brazil.
  • Larre ADS; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, Rio Grande do Sul, 90119-900, Brazil.
  • Rigotto C; State Center for Health Surveillance, Rio Grande do Sul State Health Department, Porto Alegre, Rio Grande do Sul, 90119-900, Brazil.
  • Campos FS; FEEVALE University, ERS 239 n° 2755, Novo Hamburgo, RS, 93352-000, Brazil.
  • Franco AC; Virology Laboratory, Institute for Basic Health Sciences, Federal University of Rio Grande do Sul, Brazil.
Sci One Health ; 1: 100008, 2022 Nov.
Article en En | MEDLINE | ID: mdl-39076600
ABSTRACT
Wastewater-based epidemiology (WBE) may be successfully used to comprehensively monitor and determine the scale and dynamics of some infections in the community. We monitored severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) RNA in raw wastewater samples from Porto Alegre, Southern Brazil. The samples were collected and analyzed every week between May 2020 to May 2021. Meanwhile, different social restrictions were applied according to the number of hospitalized patients in the region. Weekly samples were obtained from two wastewater treatment plants (WWTP), named Navegantes and Serraria. To determine the SARS-CoV-2 RNA titers in wastewater, we performed RT-qPCR analysis targeting the N gene (N1). The highest titer of SARS-CoV-2 RNA was observed between epidemiological weeks (EWs) 33-37 (August), 42-43 (October), 45-46 (November), 49-51 (December) in 2020, and 1-3 (January), 7-13 (February to March) in 2021, with viral loads ranging from 1 × 106-3 × 106 genomic copies/Liter. An increase in positive confirmed cases followed such high viral loads. Depending on the sampling method used, positive cases increased in 6-7 days and 15 days after the rise of viral RNA titers in wastewater, with composite sampling methods showing a lower time lag and a higher resolution on the analyses. The results showed a direct relation between strict social restrictions and the loads of detected RNA reduction in wastewater, corroborating the number of confirmed cases. Differences in viral loads between different sampling points and methods were observed, as composite samples showed more stable results during the analyzed period. Besides, viral loads obtained from samples collected at Serraria WWTP were consistently higher than the ones obtained at Navegantes WWTP, indicating differences in local dynamics of SARS-CoV-2 spread in different regions of Porto Alegre. In conclusion, wastewater sampling to monitor SARS-CoV-2 is a robust tool to evaluate the viral loads contributing to hospitalized patients' data and confirmed cases. In addition, SARS-CoV-2 detection in sewage may inform and alert the government when there are asymptomatic or non-tested patients.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE País/Región como asunto: America do sul / Brasil Idioma: En Revista: Sci One Health Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE País/Región como asunto: America do sul / Brasil Idioma: En Revista: Sci One Health Año: 2022 Tipo del documento: Article País de afiliación: Brasil Pais de publicación: Países Bajos