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From Molecules to Classrooms: A Comprehensive Guide to Single-Molecule Localization Microscopy.
Yan, Guangjie; Zhang, Yuteng; Allamprese, Alexis; Brooks, Kameron N; Chen, Wenkai; Yan, Shudan; Chen, Tai-Yen.
Afiliación
  • Yan G; Department of Chemistry, University of Houston, Houston, Texas 77204.
  • Zhang Y; Department of Chemistry, University of Houston, Houston, Texas 77204.
  • Allamprese A; Department of Chemistry, University of Houston, Houston, Texas 77204.
  • Brooks KN; Department of Chemistry, University of Houston, Houston, Texas 77204.
  • Chen W; Department of Chemistry, University of Houston, Houston, Texas 77204.
  • Yan S; Department of Chemistry, University of Houston, Houston, Texas 77204.
  • Chen TY; Department of Chemistry, University of Houston, Houston, Texas 77204.
J Chem Educ ; 101(2): 514-520, 2024 Feb 13.
Article en En | MEDLINE | ID: mdl-39070090
ABSTRACT
Single-molecule localization microscopy (SMLM) has revolutionized our ability to visualize cellular structures, offering unprecedented detail. However, the intricate biophysical principles that underlie SMLM can be daunting for newcomers, particularly undergraduate and graduate students. To address this challenge, we introduce the fundamental concepts of SMLM, providing a solid theoretical foundation. In addition, we have developed an intuitive graphical interface APP that simplifies these core concepts, making them more accessible for students. This APP clarifies how super-resolved images are fitted and highlights the crucial factors determining image quality. Our approach deepens students' understanding of SMLM by combining theoretical instruction with practical learning. This development equips them with the skills to carry out single-molecule super-resolved experiments and explore the microscopic world beyond the diffraction limit.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Chem Educ Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Chem Educ Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos