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Characterization and Genomic Analysis of Affinirhizobium gouqiense sp. nov. Isolated from Seawater of Gouqi Island Located in the East China Sea and Reclassification of Rhizobium lemnae to the Genus Affinirhizobium as Affinirhizobium lemnae comb. nov.
He, Min; Chen, Gen; Li, Ke-Jing; Tang, Xing-Xing; Liu, Xiao-Xiao; Ren, Chang-Bin; Liu, Hou-Hong; Luo, Hai; Debnath, Sanjit Chandra; Wang, Pin-Mei; Chen, Hai-Xin; Zheng, Dao-Qiong.
Afiliación
  • He M; Hainan Institute, Zhejiang University, Sanya, 572000, China.
  • Chen G; Hainan Institute, Zhejiang University, Sanya, 572000, China.
  • Li KJ; Ocean College, Zhejiang University, Zhoushan, 316021, China.
  • Tang XX; Ocean College, Zhejiang University, Zhoushan, 316021, China.
  • Liu XX; Hainan Institute, Zhejiang University, Sanya, 572000, China.
  • Ren CB; Hainan Institute, Zhejiang University, Sanya, 572000, China.
  • Liu HH; Hainan Institute, Zhejiang University, Sanya, 572000, China.
  • Luo H; Hainan Institute, Zhejiang University, Sanya, 572000, China.
  • Debnath SC; Ocean College, Zhejiang University, Zhoushan, 316021, China.
  • Wang PM; Biosciences, University of Exeter, Exeter, Geoffrey Pope Building, Devon, EX4 4HB, UK.
  • Chen HX; Ocean College, Zhejiang University, Zhoushan, 316021, China.
  • Zheng DQ; BGI Research, Sanya, 572025, China. chenhaixin@genomics.cn.
Curr Microbiol ; 81(9): 283, 2024 Jul 27.
Article en En | MEDLINE | ID: mdl-39066927
ABSTRACT
A novel bacterium designated as SSA5.23T was isolated from seawater. Cells of SSA5.23T are Gram-stain-negative, short, rod-shaped, and exhibit motility via numerous peritrichous flagella. The strain could grow at temperatures ranging from 15 to 35 °C (optimum at 25 °C), in a salinity range of 0-5.0% (w/v) NaCl, and within a pH range of 6.0-9.0 (optimum at pH 7.0). The predominant cellular fatty acid of SSA5.23T was C181 ω7c/C181 ω6c, and the major respiratory quinones were Q-9 and Q-10. Diphosphatidylglycerol, phosphatidylethanolamine, and phosphatidylglycerol were identified as the primary polar lipids. The complete genome (5.47 Mb) of SSA5.23T comprises of a circular chromosome of 3.64 Mb and three plasmids, specifically sized at 59.73 kb, 227.82 kb, and 1.54 Mb, respectively. Certain genes located on the plasmids play roles in denitrification, oxidative stress resistance, and osmotic tolerance, which likely contribute to the adaptability of this strain in marine conditions. Core-proteome average amino acid identity analysis effectively identified the strain's affiliation with the genus Affinirhizobium, showing the highest value (89.9%) with Affinirhizobium pseudoryzae DSM 19479T. This classification was further supported by the phylogenetic analysis of concatenated alignment of 170 single-copy orthologous proteins. When compared to related reference strains, SSA5.23T displayed an average nucleotide identity ranging from 74.9 to 80.3% and digital DNA-DNA hybridization values ranging from 19.9 to 23.9%. Our findings confirmed that strain SSA5.23T represents a novel species of the genus Affinirhizobium, for which the name Affinirhizobium gouqiense sp. nov. (type strain SSA5.23T = LMG 32560T = MCCC 1K07165T) was suggested.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Agua de Mar / ADN Bacteriano / Genoma Bacteriano / Ácidos Grasos País/Región como asunto: Asia Idioma: En Revista: Curr Microbiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Filogenia / Agua de Mar / ADN Bacteriano / Genoma Bacteriano / Ácidos Grasos País/Región como asunto: Asia Idioma: En Revista: Curr Microbiol Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Estados Unidos