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Digestive Profiles of Human Milk, Recombinant Human and Bovine Lactoferrin: Comparing the Retained Intact Protein and Peptide Release.
Kim, Bum Jin; Kuhfeld, Russell F; Haas, Joanna L; Anaya, Yanisa M; Martinez, Raysa Rosario; Sah, Baidya Nath P; Breen, Bella; Newsham, Kahler; Malinczak, Carrie-Anne; Dallas, David C.
Afiliación
  • Kim BJ; Nutrition Program, School of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR 97331, USA.
  • Kuhfeld RF; Nutrition Program, School of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR 97331, USA.
  • Haas JL; Department of Food Science and Technology, Oregon State University, Corvallis, OR 97331, USA.
  • Anaya YM; Helaina, New York, NY 10010, USA.
  • Martinez RR; Helaina, New York, NY 10010, USA.
  • Sah BNP; Nutrition Program, School of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR 97331, USA.
  • Breen B; Helaina, New York, NY 10010, USA.
  • Newsham K; Helaina, New York, NY 10010, USA.
  • Malinczak CA; Helaina, New York, NY 10010, USA.
  • Dallas DC; Nutrition Program, School of Nutrition and Public Health, College of Health, Oregon State University, Corvallis, OR 97331, USA.
Nutrients ; 16(14)2024 Jul 21.
Article en En | MEDLINE | ID: mdl-39064803
ABSTRACT
Lactoferrin (LF) is a major component of human milk. LF supplementation (currently bovine) supports the immune system and helps maintain iron homeostasis in adults. No recombinant human lactoferrin (rhLF) is available for commercial food use. To determine the extent to which rhLF (Effera™) produced by Komagataella phaffii digests similarly to hmLF, a validated in vitro digestion protocol was carried out. Bovine LF (bLF) was used as an additional control, as it is approved for use in various food categories. This study compared the extent of intact protein retention and the profile of peptides released in hmLF, bLF and rhLF (each with low and high iron saturation) across simulated adult gastric and intestinal digestion using gel electrophoresis, ELISA and LC-MS. Intact LF retention across digestion was similar across LF types, but the highest iron-saturated hmLF had greater retention in the simulated gastric fluid than all other sample types. Peptides identified in digested hmLF samples strongly correlated with digested rhLF samples (0.86 < r < 0.92 in the gastric phase and 0.63 < r < 0.70 in the intestinal phase), whereas digested bLF samples were significantly different. These findings support the potential for rhLF as a food ingredient for human consumption.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Digestión / Lactoferrina / Leche Humana Límite: Animals / Humans Idioma: En Revista: Nutrients Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Proteínas Recombinantes / Digestión / Lactoferrina / Leche Humana Límite: Animals / Humans Idioma: En Revista: Nutrients Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Suiza