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Phytochemical Analysis of Centaurea calcitrapa L. Aerial Flowering Parts Serial Solvent Extracts and Its Antibacterial and Antioxidant Activities.
Mekky, Alsayed E; Saied, Ebrahim; Abdelmouty, Eslam S; Haggag, Muhammad I; Khedr, Mohamed; Khalel, Ashjan F; Al-Habibi, Mahmoud M; Metwally, Shimaa A; El Askary, Ahmad; Mohammad, Abeer Mahmoud; Alshehri, Wafa A; Sharahili, Ahmed I; Khairy, Nehal M; Abdelaziz, Ahmed E M; Mahmoud, Nashaat N.
Afiliación
  • Mekky AE; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
  • Saied E; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
  • Abdelmouty ES; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
  • Haggag MI; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
  • Khedr M; Botany and Microbiology Department, Faculty of Science, Al-Azhar University, Cairo 11884, Egypt.
  • Khalel AF; Biology Department, Al-Darb University College, Jazan University, Jazan 45142, Saudi Arabia.
  • Al-Habibi MM; Microbiology and Immunology Department, Faculty of Pharmacy (Boys), Al-Azhar University, Cairo 11884, Egypt.
  • Metwally SA; Microbiology and Immunology Department, Faculty of Pharmacy (Girls), Al-Azhar University, Cairo 11884, Egypt.
  • El Askary A; Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, Taif University, P.O. Box 11099, Taif 21944, Saudi Arabia.
  • Mohammad AM; Biology Department, Al-Darb University College, Jazan University, Jazan 45142, Saudi Arabia.
  • Alshehri WA; Department of Biological Sciences, College of Science, University of Jeddah, Jeddah 23890, Saudi Arabia.
  • Sharahili AI; Department of Clinical Laboratories, Medical Biochemistry Unit, Najran General Hospital, Najran 66277, Saudi Arabia.
  • Khairy NM; Ministry of Health, Riyadh 12613, Saudi Arabia.
  • Abdelaziz AEM; Department of Microbiology and Immunology, Egypt Drug Authority (EDA), (Formerly NODCAR), Giza 12654, Egypt.
  • Mahmoud NN; Department of Microbiology and Immunology, Faculty of Pharmacy, Sinai University-East Kantara Branch, Ismailia 41636, Egypt.
Life (Basel) ; 14(7)2024 Jul 19.
Article en En | MEDLINE | ID: mdl-39063653
ABSTRACT
To evaluate the phytochemical composition, antibacterial, and antioxidant activity of successive extracts of Centaurea calcitrapa L. (C. calcitrapa) aerial flowering parts, they were assessed in vitro. Using a spectrophotometer, the sample absorbance at 517 nm was used to quantify the scavenging activity. The negative control was DPPH. In the current study, the diffusion using agar wells technique was adapted to measure antimicrobial activity. Phytochemical analysis was performed using the recommended standard procedures. The methanol extract of C. calcitrapa exhibited high levels of total phenolic acids expressed as gallic acid (GA), measured as (97.25 ± 0.73 mg GAE/g) content compared to the chloroform, acetyl acetate, and aqueous extracts (27.42 ± 0.29, 64.25 ± 0.96, and 17.25 ± 0.73 mg GAE/g), respectively. Additionally, the methanol extract had a higher total tannin (27.52 ± 0.53 mg TAE/g) content compared to the chloroform, ethyl acetate, and aqueous extracts (12.02 ± 0.55, 26.01 ± 0.81, and 7.35 ± 0.56 mg TAE/g), respectively, while the aqueous extract contains a lower percentage of flavonoids (141.10 ± 1.31 mg RTE/g) compared to the higher content achieved by the methanol extract (425.93 ± 1.27 mg RTE/g). The hydroxyl groups of the flavonoid and the phenolic compounds found in C. calcitrapa are essentially scavenging free radicals. Radical scavenging activity was highest in the methanol extract (IC50 = 2.82 µg/mL), aqueous extract (IC50 = 8.03 µg/mL), ethyl acetate extract (IC50 = 4.79 µg/mL), and chloroform extract (IC50 = 6.33 µg/mL), as compared to the standard scavenging activity (IC50 = 2.52 µg/mL). The antibacterial properties of C. calcitrapa against Gram-negative bacterial strains Klebsiella pneumoniae, Escherichia coli, Enterobacter aerogenes, and Acinetobacter baumanii, in addition to Gram-positive strains Staphylococcus haemolyticus, Enterococcus faecalis, and Staphylococcus aureus, revealed inhibition zone diameter. The findings of this investigation establish that the aerial flowering parts of C. calcitrapa have substantial antibacterial action against human infections, and the plant can serve as a significant antioxidant that can be employed to prevent and treat severe degenerative diseases brought on by oxidative stress. qPCR showed that C. calcitrapa extracts elevate both SOD1 and SOD2 (cellular oxidation markers) with remarkable folds (1.8-fold for SOD1 and SOD2) with ethyl acetate plant extract against ascorbic acid as a control. This result reflects that C. calcitrapa extracts have remarkable antioxidant activity.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Life (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Life (Basel) Año: 2024 Tipo del documento: Article País de afiliación: Egipto Pais de publicación: Suiza