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Optimised stress - intensification of pyocyanin production with zinc oxide nanoparticles.
Honselmann Genannt Humme, Joanna; Dubrowska, Kamila; Grygorcewicz, Bartlomiej; Glizniewicz, Marta; Paszkiewicz, Oliwia; Glowacka, Anna; Musik, Daniel; Story, Grzegorz; Rakoczy, Rafal; Augustyniak, Adrian.
Afiliación
  • Honselmann Genannt Humme J; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, Szczecin, 71-065, Poland. joanna_jablonska@zut.edu.pl.
  • Dubrowska K; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, Szczecin, 71-065, Poland.
  • Grygorcewicz B; Department of Forensic Genetics, Faculty of Medicine and Dentistry, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, Szczecin, 70-111, Poland.
  • Glizniewicz M; Department of Forensic Genetics, Faculty of Medicine and Dentistry, Pomeranian Medical University in Szczecin, Powstanców Wielkopolskich 72, Szczecin, 70-111, Poland.
  • Paszkiewicz O; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, Szczecin, 71-065, Poland.
  • Glowacka A; Department of Environmental Engineering, Faculty of Civil and Environmental Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 50a, Szczecin, 70-311, Poland.
  • Musik D; Department of Environmental Engineering, Faculty of Civil and Environmental Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 50a, Szczecin, 70-311, Poland.
  • Story G; ESC Global, Sp. z o.o., Sloneczny Sad 4F, 72-002, Doluje, Poland.
  • Rakoczy R; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, Szczecin, 71-065, Poland.
  • Augustyniak A; Department of Chemical and Process Engineering, Faculty of Chemical Technology and Engineering, West Pomeranian University of Technology in Szczecin, Piastów Avenue 42, Szczecin, 71-065, Poland.
Microb Cell Fact ; 23(1): 215, 2024 Jul 27.
Article en En | MEDLINE | ID: mdl-39061071
ABSTRACT

BACKGROUND:

Pyocyanin is a blue pigment produced by Pseudomonas aeruginosa. Due to its unique redox properties over the last decade, it has gained more and more interest as a utile chemical. Nevertheless, it remains a rather costly reagent. It was previously shown that the production of pyocyanin can be enhanced by employing various methods. Among them are using statistical methods for planning the experiments or exposing bacterial cultures to stressors such as nanoparticles dosed in sublethal concentrations, e.g. zinc oxide nanoparticles.

RESULTS:

The Design of Experiment (DoE) methodology allowed for calculating the optimal process temperature and nanoparticle concentration to intensify pyocyanin production. Low concentrations of the nanoparticles (6.06 µg/mL) and a temperature of 32℃ enhanced pyocyanin production, whereas higher concentrations of nanoparticles (275.75 µg/mL) and higher temperature stimulated biomass production and caused the abolishment of pyocyanin production. Elevated pigment production in zinc oxide nanoparticles-supplemented media was sustained in the scaled-up culture. Conducted analyses confirmed that observed stimulation of pyocyanin production is followed by higher membrane potential, altered gene expression, generation of reactive oxygen species, and accumulation of zinc in the cell's biomass.

CONCLUSIONS:

Pyocyanin production can be steered using ZnO nanoparticles. Elevated production of pyocyanin due to exposure to nanoparticles is followed by the number of changes in physiology of bacteria and is a result of the cellular stress. We showed that the stress response of bacteria can be optimised using statistical methods and result in producing the desired metabolite more effectively.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Piocianina / Óxido de Zinc Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Pseudomonas aeruginosa / Piocianina / Óxido de Zinc Idioma: En Revista: Microb Cell Fact Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: Polonia Pais de publicación: Reino Unido