Your browser doesn't support javascript.
loading
Homologous Delta-12 Fatty Acid Desaturase (FAD2) Genes Affect Gene Expression and Linoleic Acid Levels in Lentinula edodes under Heat Stress.
Yang, Huanling; Jiang, Jun; Chen, Mingjie; Song, Xiaoxia; Yu, Changxia; Chen, Hongyu; Zhao, Yan.
Afiliación
  • Yang H; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Jiang J; Lishui Institute of Agriculture and Forestry Sciences, Lishui 323000, China.
  • Chen M; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Song X; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Yu C; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Chen H; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
  • Zhao Y; Institute of Edible Fungi, Shanghai Academy of Agricultural Sciences, Shanghai 201403, China.
J Fungi (Basel) ; 10(7)2024 Jul 18.
Article en En | MEDLINE | ID: mdl-39057381
ABSTRACT
Delta-12 fatty acid desaturases (FAD2s) actively regulate stress responses and cell differentiation in living organisms. In this study, six homologous FAD2 genes were identified based on the genome sequence of Lentinula edodes. Then, the six FAD2 protein sequences were analyzed using bioinformatics tools, including ExPASy ProtParam, SignalP, TMHMM, and TargetP. These analyses were performed to predict the physical and chemical properties, signal peptides, and transmembrane and conserved domains of these proteins. The polypeptide sequences were aligned, and a maximum likelihood phylogenetic tree was constructed using MEGA 7.0 software to elucidate the phylogenetic relationships between homologous FAD2 sequences. The results demonstrated that the FAD2 proteins contained three conserved histidine-rich regions (HXXXH, HXXHH, and HXXHH), which included eight histidine residues. The linoleic acid content and FAD2 enzyme activity were further analyzed, and the levels in the mutagenic heat-tolerant strain 18N44 were lower than those in the wild-type strain 18. Interestingly, the expression levels of the FAD2-2 and FAD2-3 genes under heat stress in strain 18N44 were lower than those in strain 18. These findings indicated that FAD2-2 and FAD2-3 may play major roles in the synthesis of linoleic acid during heat stress.
Palabras clave
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: J Fungi (Basel) Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: Suiza