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Expression profile and gap-junctional transfer of microRNAs in the bovine cumulus-oocyte complex.
Six, R; Benedetti, C; Fan, Y; Guan, X; Gansemans, Y; Hedia, Mohamed; Bogado Pascottini, O; Pavani, K C; Van Nieuwerburgh, F; Deforce, D; Smits, K; Van Soom, A; Peelman, L.
Afiliación
  • Six R; Department of Veterinary and Biosciences, Ghent University, Merelbeke, Belgium.
  • Benedetti C; Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
  • Fan Y; Department of Veterinary and Biosciences, Ghent University, Merelbeke, Belgium.
  • Guan X; Department of Veterinary and Biosciences, Ghent University, Merelbeke, Belgium.
  • Gansemans Y; Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
  • Hedia M; Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
  • Bogado Pascottini O; Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt.
  • Pavani KC; Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
  • Van Nieuwerburgh F; Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
  • Deforce D; Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
  • Smits K; Laboratory of Pharmaceutical Biotechnology, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
  • Van Soom A; Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
  • Peelman L; Department of Internal Medicine, Reproduction and Population Medicine, Ghent University, Merelbeke, Belgium.
Front Cell Dev Biol ; 12: 1404675, 2024.
Article en En | MEDLINE | ID: mdl-39055654
ABSTRACT
MicroRNAs (miRNA) are important regulators of oocyte maturation, playing a key role in modulating gene expression both in a temporal- and spatial-specific manner. These small non-coding RNAs are involved in important processes during oocyte maturation, acting as messengers between the oocyte and its surrounding cumulus cells. Despite its significance, the bidirectional communication mechanism is still unknown. To test miRNA communication between oocyte and surrounding cumulus cells through the gap junctions the gap junctions were either blocked with carbenoxolone or not. MiRNA sequencing of oocytes at 1, 6, and 22 h of in vitro maturation was then performed. Among the differentially expressed miRNAs, bta-miR-21-5p, a regulator of cumulus cell viability and oocyte maturation, was the only previously known miRNA. Furthermore, by labeling a bta-miR-21-5p mimic with FAM, crossing of this miRNA through the gap junctions within the cumulus-oocyte complex could be visualized and internalization in the oocyte was confirmed by RT-qPCR. In conclusion, this study provides, for the first time, evidence that miRNA communication within the bovine cumulus-oocyte complex is enabled through the gap junctional network.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Dev Biol Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Cell Dev Biol Año: 2024 Tipo del documento: Article País de afiliación: Bélgica Pais de publicación: Suiza