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Molecular Background of RhD-positive and RhD-negative Phenotypes in a Saudi Population.
Alalshaikh, Mohrah A; Alsughayir, Ammar H; Alsaif, Alyazeed S; Ababtain, Sarah A; Aloyouni, Shaika Y; Aldilaijan, Khawlah E; Alsubaie, Sahar F.
Afiliación
  • Alalshaikh MA; Department of Clinical Laboratory Sciences, Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
  • Alsughayir AH; King Fahad Medical City, The Saudi Society of Blood and Marrow Transplantation, Riyadh, Saudi Arabia.
  • Alsaif AS; Transfusion Medicine and Blood Bank Department, King Fahad Medical City, Riyadh, Saudi Arabia.
  • Ababtain SA; Department of Research, Health Sciences Research Centre, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
  • Aloyouni SY; Department of Research, Health Sciences Research Centre, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
  • Aldilaijan KE; Department of Research, Health Sciences Research Centre, Princess Nourah Bint Abdulrahman University, Riyadh, Saudi Arabia.
  • Alsubaie SF; Department of Clinical Laboratory Sciences, Applied Medical Sciences, King Saud University, Riyadh, Saudi Arabia.
Saudi J Med Med Sci ; 12(3): 210-215, 2024.
Article en En | MEDLINE | ID: mdl-39055072
ABSTRACT

Background:

The RHD gene is one of the most complex blood group genes. The molecular background of the RHD gene in RhD-negative and RhD-positive individuals varies within and among different populations. Knowing the molecular basis of the RHD gene in a specific population is required to establish effective genotyping methods. While the molecular basis has been revealed in many ethnicities, such as Caucasians and Black Africans, it still requires elucidation in Arabs.

Objectives:

The aim of this study was to gain insights into the molecular basis of RhD-positive and RhD-negative phenotypes in Saudi donors. Materials and

Methods:

Conventional serological tests were used to determine the Rh phenotypes in 136 Saudi donors by typing D, C, c, E, and e antigens. Multiplex-PCR and Single Specific Primer-PCR were used to detect the presence of exons 3, 4, and 7 and the hybrid Rhesus box gene, respectively, in RhD-negative and/or RhD-positive samples.

Results:

Of the 136 samples, 70 were RhD positive and 66 were RhD negative. None of the RhD-negative donors had any of the three tested exons, whereas the hybrid Rhesus box gene was detected in all, indicating the zygosity status of the RHD deletion allele. The hybrid Rhesus box gene was detected in 79% of the RhD-positive individuals, suggesting high frequencies of RHD-negative haplotypes.

Conclusions:

The study findings indicate that Saudis with the RhD-negative phenotype are likely to have an entire RHD deletion in the homozygous state. However, a more comprehensive analysis of variant RHD alleles in the Saudi population is required to implement effective and dedicated molecular RHD typing strategies.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Saudi J Med Med Sci Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: India
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Saudi J Med Med Sci Año: 2024 Tipo del documento: Article País de afiliación: Arabia Saudita Pais de publicación: India