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Empowering the on-site detection of nucleic acids by integrating CRISPR and digital signal processing.
Lee, Chang Yeol; Kim, Hyunho; Degani, Ismail; Lee, Hanna; Sandoval, Angel; Nam, Yoonho; Pascavis, Madeleine; Park, Hyun Gyu; Randall, Thomas; Ly, Amy; Castro, Cesar M; Lee, Hakho.
Afiliación
  • Lee CY; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
  • Kim H; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
  • Degani I; Bionanotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea.
  • Lee H; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
  • Sandoval A; Department of Radiology, Massachusetts General Hospital, Harvard Medical School, Boston, MA, USA.
  • Nam Y; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
  • Pascavis M; Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, MA, USA.
  • Park HG; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
  • Randall T; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
  • Ly A; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
  • Castro CM; Department of Chemical and Biomolecular Engineering (BK21 Four), Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-gu, Daejeon, 34141, Republic of Korea.
  • Lee H; Center for Systems Biology, Massachusetts General Hospital Research Institute, Boston, MA, USA.
Nat Commun ; 15(1): 6271, 2024 Jul 25.
Article en En | MEDLINE | ID: mdl-39054353
ABSTRACT
Addressing the global disparity in cancer care necessitates the development of rapid and affordable nucleic acid (NA) testing technologies. This need is particularly critical for cervical cancer, where molecular detection of human papillomavirus (HPV) has emerged as an accurate screening method. However, implementing this transition in low- and middle-income countries has been challenging due to the high costs and centralized facilities required for current NA tests. Here, we present CreDiT (CRISPR Enhanced Digital Testing) for on-site NA detection. The CreDiT platform integrates i) a one-pot CRISPR strategy that simultaneously amplifies both target NAs and analytical signals and ii) a robust fluorescent detection based on digital communication (encoding/decoding) technology. These features enable a rapid assay (<35 minutes) in a single streamlined workflow. We demonstrate the sensitive detection of cell-derived HPV DNA targets down to single copies and accurate identification of HPV types in clinical cervical brushing specimens (n = 121).
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus Límite: Female / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Neoplasias del Cuello Uterino / Infecciones por Papillomavirus Límite: Female / Humans Idioma: En Revista: Nat Commun Asunto de la revista: BIOLOGIA / CIENCIA Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Reino Unido