The ubiquitin-proteasome system degrades fatty acid synthase under nitrogen starvation when autophagy is dysfunctional in Saccharomyces cerevisiae.

Jang, Hae-Soo; Lee, Yongook; Kim, Yeonsoo; Huh, Won-Ki

Jang, Hae-Soo; Lee, Yongook; Kim, Yeonsoo; Huh, Won-Ki.

Afiliación

Jang HS; School of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Lee Y; School of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Kim Y; School of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea.
Huh WK; School of Biological Sciences, Seoul National University, Seoul, 08826, Republic of Korea; Institute of Microbiology, Seoul National University, Seoul, 08826, Republic of Korea. Electronic address: wkh@snu.ac.kr.

Biochem Biophys Res Commun ; 733: 150423, 2024 11 12.

Article en En | MEDLINE | ID: mdl-39053108

ABSTRACT

ABSTRACT

Autophagy and the ubiquitin-proteasome system (UPS) are two major protein quality control mechanisms maintaining cellular proteostasis. In Saccharomyces cerevisiae, the de novo synthesis of saturated fatty acids is performed by a multienzyme complex known as fatty acid synthase (FAS). A recent study reported that yeast FAS is preferentially degraded by autophagy under nitrogen starvation. In this study, we examined the fate of FAS during nitrogen starvation when autophagy is dysfunctional. We found that the UPS compensates for FAS degradation in the absence of autophagy. Additionally, we discovered that the UPS-dependent degradation of Fas2 requires the E3 ubiquitin ligase Ubr1. Our findings highlight the complementary relationship between autophagy and the UPS.

Asunto(s)

Autofagia; Nitrógeno; Complejo de la Endopetidasa Proteasomal; Proteínas de Saccharomyces cerevisiae; Saccharomyces cerevisiae; Ubiquitina-Proteína Ligasas; Ubiquitina; Saccharomyces cerevisiae/metabolismo; Complejo de la Endopetidasa Proteasomal/metabolismo; Proteínas de Saccharomyces cerevisiae/metabolismo; Proteínas de Saccharomyces cerevisiae/genética; Nitrógeno/metabolismo; Ubiquitina/metabolismo; Ubiquitina-Proteína Ligasas/metabolismo; Ubiquitina-Proteína Ligasas/genética; Proteolisis; Ácido Graso Sintasas/metabolismo; Ácido Graso Sintasas/genética

Palabras clave

Autophagy; Fatty acid synthase; Nitrogen starvation; Saccharomyces cerevisiae; Ubiquitin-proteasome system

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Saccharomyces cerevisiae / Autofagia / Proteínas de Saccharomyces cerevisiae / Ubiquitina / Ubiquitina-Proteína Ligasas / Complejo de la Endopetidasa Proteasomal / Nitrógeno Idioma: En Revista: Biochem Biophys Res Commun Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos

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