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Drug Screening Using Normal Cell and Cancer Cell Mixture in an Automated 3D Cell Culture System.
Carson, Mathew C; Xu, Peng; Gildea, John J; Marino, Collin F; Felder, Robin A.
Afiliación
  • Carson MC; Department of Pathology, University of Virginia Health System, Charlottesville, VA, USA.
  • Xu P; Charles L. Brown Department of Electrical and Computer Engineering, School of Engineering, University of Virginia, Charlottesville, VA, USA.
  • Gildea JJ; Department of Pathology, University of Virginia Health System, Charlottesville, VA, USA.
  • Marino CF; Department of Pathology, University of Virginia Health System, Charlottesville, VA, USA.
  • Felder RA; Department of Pathology, University of Virginia Health System, Charlottesville, VA, USA.
Methods Mol Biol ; 2823: 95-108, 2024.
Article en En | MEDLINE | ID: mdl-39052216
ABSTRACT
Three-dimensional (3D) cell culture creates a more physiologically relevant environment for enhanced drug screening capabilities using microcarriers. An automated 3D system that integrates robotic manipulators, liquid handling systems, sensors, and environment control systems has the capacity to handle multiple samples in parallel, perform repetitive tasks, and provide real-time monitoring and analysis. This chapter describes a potential 3D cell culture drug screening model by combining renal proximal tubule cells as a representative normal cell line with cancer cell lines. This combination is subjected to drug screening to evaluate the drug's efficacy in suppressing cancer cells while minimizing impact on normal cells with the added benefit of having the ability to separate the two cell types by magnetic isolation for high content screens including mass spectrometry-based proteomics. This study presents advancements in 3D cell culture techniques, emphasizing the importance of automation and the potential of microcarriers in drug screening and disease modeling.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo Tridimensional de Células Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnicas de Cultivo Tridimensional de Células Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos