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[Baicalin suppresses type 2 dengue virus-induced autophagy of human umbilical vein endothelial cells by inhibiting the PI3K/AKT pathway].
Cheng, Y; Wang, Y; Yao, F; Hu, P; Chen, M; Wu, N.
Afiliación
  • Cheng Y; Laboratory of Chemistry and Biochemistry, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
  • Wang Y; Laboratory of Chemistry and Biochemistry, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
  • Yao F; Laboratory of Chemistry and Biochemistry, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
  • Hu P; Laboratory of Chemistry and Biochemistry, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
  • Chen M; Laboratory of Chemistry and Biochemistry, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
  • Wu N; Laboratory of Chemistry and Biochemistry, School of Basic Medical Sciences, Guizhou Medical University, Guiyang 561113, China.
Nan Fang Yi Ke Da Xue Xue Bao ; 44(7): 1272-1283, 2024 Jul 20.
Article en Zh | MEDLINE | ID: mdl-39051073
ABSTRACT

OBJECTIVE:

To investigate the effect of type 2 dengue virus (DENV-2) infection on autophagy in human umbilical vein endothelial cells (HUVECs) and the mechanism mediating the inhibitory effect of baicalin against DENV-2 infection.

METHODS:

Cultured HUVECs with DENV-2 infection were treated with different concentrations of baicalin, and the changes in autophagy of the cells were detected using transmission electron microscopy. Lyso Tracker Red staining was used to examine pH changes in the lysosomes of the cells, and the expressions of ATG5, beclin-1, LC3, P62, STX17, SNAP29, VAMP8, and PI3K/AKT signaling pathway-related proteins were detected by Western blotting. DENV-2 replication in the cells were evaluated using RT-qPCR. The differentially expressed proteins in DENV-2-infected HUVECs were identified by proteomics screening.

RESULTS:

Treatment with baicalin did not significantly affect the viability of cultured HUVECs. Proteomic studies suggested that the PI3K-AKT pathway played an important role in mediating cell injury induced by DENV-2 infection. The results of RT-qPCR demonstrated that baicalin dose-dependently inhibited DENV-2 replication in HUVECs and produced the strongest inhibitory effect at the concentration of 50 µg/mL. Transmission electron microscopy, Lyso Tracker Red staining, RT-qPCR, and Western blotting all showed significant inhibitory effect of baicalin on DENV-2-induced autophagy in HUVECs. DENV-2 infection of HUVECs caused increased cellular expressions of LC3 and P62 proteins, which were significantly lowered by treatment with LY294002 (a PI3K inhibitor).

CONCLUSION:

Baicalin inhibits DENV-2 replication in HUVECs and suppresses DENV-2-induced cell autophagy by inhibiting the PI3K/AKT signaling pathway.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Flavonoides / Transducción de Señal / Fosfatidilinositol 3-Quinasas / Virus del Dengue / Proteínas Proto-Oncogénicas c-akt / Células Endoteliales de la Vena Umbilical Humana Límite: Humans Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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Imprimir
XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Autofagia / Flavonoides / Transducción de Señal / Fosfatidilinositol 3-Quinasas / Virus del Dengue / Proteínas Proto-Oncogénicas c-akt / Células Endoteliales de la Vena Umbilical Humana Límite: Humans Idioma: Zh Revista: Nan Fang Yi Ke Da Xue Xue Bao Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China