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Heterogeneity of Wnt1-Cre-marked and Pax2-Cre-marked first branchial arch cranial neural crest cells in mice.
Xu, Jue; Liu, Shuang; Fu, Honggao; Shao, Meiying; Chen, Meiling; Huang, Zhen.
Afiliación
  • Xu J; West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
  • Liu S; West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
  • Fu H; College of Life Sciences, Fujian Normal University, Fuzhou 350007, China.
  • Shao M; West China School of Public Health and West China Fourth Hospital, Sichuan University, Chengdu 610041, China.
  • Chen M; College of Life Sciences, Fujian Normal University, Fuzhou 350007, China.
  • Huang Z; College of Life Sciences, Fujian Normal University, Fuzhou 350007, China.
Hua Xi Kou Qiang Yi Xue Za Zhi ; 42(4): 435-443, 2024 Aug 01.
Article en En, Zh | MEDLINE | ID: mdl-39049630
ABSTRACT

OBJECTIVES:

This study aimed to explore the heterogeneity and gene ontology of Wnt1-Cre-marked and Pax2-Cre-marked first branchial arch cranial neural crest cells (CNCs) in mice.

METHODS:

The embryos of Wnt1-Cre;R26RmTmG and Pax2-Cre;R26RmTmG at embryonic day (E)8.0-E9.25 were collected for histological observation. We performed immunostaining to compare green fluorescent protein (GFP)-positive CNCs in Pax2-Cre;R26RAi9 and Wnt1-Cre;R26RAi9 mice at E15.5. Single-cell RNA sequencing (scRNA-seq) was used to analyze the first branchial arch GFP-positive CNCs from Wnt1-Cre;R26RmTmG and Pax2-cre;R26RmTmGmice at E10.5. Real time fluorescence quantitative polymerase chain reaction (q-PCR) was performed to validate the differential genes.

RESULTS:

Wnt1-Cre-marked and Pax2-Cre-marked CNCs migrated from the neural plateto first and second branchial arches and to the first branchial arch, respectively, at E8.0. Although Wnt1-Cre-marked and Pax2-Cre-marked CNCs were found mostly in cranial-facial tissues, the former had higher expression in palate and tongue. The results of scRNA-seq showed that Pax2-Cre-marked CNCs specifically contributed to osteoblast differentiation and ossification, while Wnt1-Cre-marked CNCs participated in limb development, cell migration, and ossification. The q-PCR data also confirmed the results of gene ontology analysis.

CONCLUSIONS:

Pax2-Cre mice are perfect experimental animal models for research on first branchial arch CNCs and derivatives in osteoblast differentiation and ossification.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Región Branquial / Proteína Wnt1 / Factor de Transcripción PAX2 / Cresta Neural Límite: Animals Idioma: En / Zh Revista: Hua Xi Kou Qiang Yi Xue Za Zhi Asunto de la revista: ODONTOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China
Texto completo
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XML
PubMed Links
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Región Branquial / Proteína Wnt1 / Factor de Transcripción PAX2 / Cresta Neural Límite: Animals Idioma: En / Zh Revista: Hua Xi Kou Qiang Yi Xue Za Zhi Asunto de la revista: ODONTOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China