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Protocol for assessing GD2 on formalin-fixed paraffin-embedded tissue sections using immunofluorescence staining.
Kailayangiri, Sareetha; Altvater, Bianca; Farwick, Nicole; Meltzer, Jutta; Hartmann, Wolfgang; Rossig, Claudia.
Afiliación
  • Kailayangiri S; Department of Pediatric Hematology and Oncology, University Children's Hospital Muenster, Muenster, Germany; Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands.
  • Altvater B; Department of Pediatric Hematology and Oncology, University Children's Hospital Muenster, Muenster, Germany; Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands.
  • Farwick N; Department of Pediatric Hematology and Oncology, University Children's Hospital Muenster, Muenster, Germany.
  • Meltzer J; Department of Pediatric Hematology and Oncology, University Children's Hospital Muenster, Muenster, Germany.
  • Hartmann W; Gerhard-Domagk-Institute of Pathology, University of Muenster, Muenster, Germany.
  • Rossig C; Department of Pediatric Hematology and Oncology, University Children's Hospital Muenster, Muenster, Germany; Princess Máxima Center for Pediatric Oncology, Utrecht, the Netherlands; Institute of Transfusion Medicine and Cell Therapy, University Hospital Muenster, Muenster, Germany; Cells-in-Motion C
STAR Protoc ; 5(3): 103199, 2024 Sep 20.
Article en En | MEDLINE | ID: mdl-39046881
ABSTRACT
The detection of disialoganglioside GD2 on tumor biopsies, especially in paraffin-embedded tissues, has been challenging due to the glycolipid structure of GD2 and its membrane anchorage. Here, we present an immunofluorescence protocol for the reliable assessment of GD2 on formalin-fixed paraffin-embedded (FFPE) tissues. We describe steps for antigen retrieval with Tris-EDTA buffer and staining with unconjugated anti-GD2 antibody (clone 14.G2a) and horse radish peroxidase (HRP)-conjugated secondary antibody. We then detail procedures for signal amplification using the tyramide signal amplification technique. For complete details on the use and execution of this protocol, please refer to Fischer-Riepe et al.1.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnica del Anticuerpo Fluorescente / Adhesión en Parafina / Formaldehído Límite: Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Técnica del Anticuerpo Fluorescente / Adhesión en Parafina / Formaldehído Límite: Humans Idioma: En Revista: STAR Protoc Año: 2024 Tipo del documento: Article País de afiliación: Países Bajos Pais de publicación: Estados Unidos