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The methane-oxidizing microbial communities of three maar lakes in tropical monsoon Asia.
Bicaldo, Iona Eunice C; Padilla, Karol Sophia Agape R; Tu, Tzu-Hsuan; Chen, Wan Ting; Mendoza-Pascual, Milette U; Vicera, Carmela Vannette B; de Leon, Justine R; Poblete, Kamille N; Austria, Eleanor S; Lopez, Mark Louie D; Kobayashi, Yuki; Shiah, Fuh-Kwo; Papa, Rey Donne S; Okuda, Noboru; Wang, Pei-Ling; Lin, Li-Hung.
Afiliación
  • Bicaldo IEC; The Graduate School, University of Santo Tomas, Manila, Philippines.
  • Padilla KSAR; Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, Philippines.
  • Tu TH; The Graduate School, University of Santo Tomas, Manila, Philippines.
  • Chen WT; Philippine Genome Center, University of the Philippines, Quezon City, Philippines.
  • Mendoza-Pascual MU; Department of Science and Technology, Science Education Institute, Taguig, Philippines.
  • Vicera CVB; Department of Geosciences, National Taiwan University, Taipei, Taiwan.
  • de Leon JR; Department of Oceanography, National Sun Yat-sen University, Kaohsiung, Taiwan.
  • Poblete KN; Department of Geosciences, National Taiwan University, Taipei, Taiwan.
  • Austria ES; Department of Environmental Science, School of Science and Engineering, Ateneo Research Institute for Science and Engineering, Ateneo de Manila University, Quezon City, Philippines.
  • Lopez MLD; Department of Biological Sciences, University of Santo Tomas, Manila, Philippines.
  • Kobayashi Y; Research Center for the Natural and Applied Sciences, University of Santo Tomas, Manila, Philippines.
  • Shiah FK; Department of Biological Sciences, University of Santo Tomas, Manila, Philippines.
  • Papa RDS; The Graduate School, University of Santo Tomas, Manila, Philippines.
  • Okuda N; Biology Department, Adamson University, Manila, Philippines.
  • Wang PL; Department of Biochemistry and Microbiology, University of Victoria, Victoria, BC, Canada.
  • Lin LH; Center for Ecological Research, Kyoto University, Shiga, Japan.
Front Microbiol ; 15: 1410666, 2024.
Article en En | MEDLINE | ID: mdl-39044952
ABSTRACT
Methane-oxidizing bacteria (MOB) is a group of planktonic microorganisms that use methane as their primary source of cellular energy. For tropical lakes in monsoon Asia, there is currently a knowledge gap on MOB community diversity and the factors influencing their abundance. Herewith, we present a preliminary assessment of the MOB communities in three maar lakes in tropical monsoon Asia using Catalyzed Reporter Deposition, Fluorescence In-Situ Hybridization (CARD-FISH), 16S rRNA amplicon sequencing, and pmoA gene sequencing. Correlation analysis between MOB abundances and lakes' physicochemical parameters following seasonal monsoon events were performed to explain observed spatial and temporal patterns in MOB diversity. The CARD-FISH analyses detected the three MOB types (I, II, and NC10) which aligned with the results from 16S rRNA amplicons and pmoA gene sequencing. Among community members based on 16S rRNA genes, Proteobacterial Type I MOB (e.g., Methylococcaceae and Methylomonadaceae), Proteobacterial Type II (Methylocystaceae), Verrucomicrobial (Methylacidiphilaceae), Methylomirabilota/NC10 (Methylomirabilaceae), and archaeal ANME-1a were found to be the dominant methane-oxidizers in three maar lakes. Analysis of microbial diversity and distribution revealed that the community compositions in Lake Yambo vary with the seasons and are more distinct during the stratified period. Temperature, DO, and pH were significantly and inversely linked with type I MOB and Methylomirabilota during stratification. Only MOB type I was influenced by monsoon changes. This research sought to establish a baseline for the diversity and ecology of planktonic MOB in tropical monsoon Asia to better comprehend their contribution to the CH4 cycle in tropical freshwater ecosystems.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Filipinas Pais de publicación: Suiza
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Front Microbiol Año: 2024 Tipo del documento: Article País de afiliación: Filipinas Pais de publicación: Suiza