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[EMP3 inhibits the formation of heterotypic cell-in-cell structures in hepatocellular carcinoma].
Zhang, Yangyi; Wang, Chenxi; Feng, Pengfei; Liu, Chenyu; Ren, He; Yang, Yalan; Huang, Yinuo; Sun, Qiang; Huang, Hongyan.
Afiliación
  • Zhang Y; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China.
  • Wang C; Research Unit of Cell Death Mechanism, Chinese Academy of Medical Sciences, Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
  • Feng P; Research Unit of Cell Death Mechanism, Chinese Academy of Medical Sciences, Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
  • Liu C; Research Unit of Cell Death Mechanism, Chinese Academy of Medical Sciences, Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
  • Ren H; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China.
  • Yang Y; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China.
  • Huang Y; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China.
  • Sun Q; Department of Oncology, Beijing Shijitan Hospital of Capital Medical University, Beijing 100038, China.
  • Huang H; Research Unit of Cell Death Mechanism, Chinese Academy of Medical Sciences, Beijing Institute of Biotechnology, Academy of Military Medical Sciences, Beijing 100071, China.
Sheng Wu Gong Cheng Xue Bao ; 40(7): 2223-2234, 2024 Jul 25.
Article en Zh | MEDLINE | ID: mdl-39044586
ABSTRACT
Heterotypic cell-in-cell (heCIC) structures represent a unique intercellular interaction where tumor cells internalize immune cells to enhance the killing efficiency of immune cells. However, the mechanism of heCIC structure formation remains to be fully elucidated. In this study, we explored the role of epithelial membrane protein 3 (EMP3), a PMP-22/EMP/MP20 protein family member highly expressed in the patients with hepatocellular carcinoma and poor prognosis, in the formation of the heCIC structure formed by natural killer cells and hepatocellular carcinoma cells. The analysis of monoclonal hepatocellular carcinoma cell lines revealed that EMP3 presented low expression in the cells with high capability to form heCIC structure and high expression in those with low capability. Knocking down the expression of EMP3 by gene editing promoted the formation of heCIC structures, while overexpression of EMP3 significantly inhibited this process. Additionally, the expression of factors involved in the heCIC structure formation suggested that EMP3 inhibited the formation of heCIC structures by modulating the adhesion ability and cytoskeleton of tumor cells. The findings lay a foundation for enhancing the heCIC-mediated tumor immunotherapy by targeting EMP3.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Células Asesinas Naturales / Adhesión Celular / Carcinoma Hepatocelular / Neoplasias Hepáticas Límite: Humans Idioma: Zh Revista: Sheng Wu Gong Cheng Xue Bao Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Glicoproteínas de Membrana / Células Asesinas Naturales / Adhesión Celular / Carcinoma Hepatocelular / Neoplasias Hepáticas Límite: Humans Idioma: Zh Revista: Sheng Wu Gong Cheng Xue Bao Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China