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[Progress in research and application of the biotin ligase BirA and its mutants in pathogen-host interaction].
Zhang, Hanyu; Li, Pengfei; Liao, Zheyu; Zhang, Mengjia; Sun, Yumei; Zhang, Min; He, Qigai; Li, Wentao.
Afiliación
  • Zhang H; National Key Laboratory of Agricultural Microbiology, Wuhan 430070, Hubei, China.
  • Li P; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
  • Liao Z; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs, Wuhan 430070, Hubei, China.
  • Zhang M; National Key Laboratory of Agricultural Microbiology, Wuhan 430070, Hubei, China.
  • Sun Y; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
  • Zhang M; Key Laboratory of Development of Veterinary Diagnostic Products, Ministry of Agriculture and Rural Affairs, Wuhan 430070, Hubei, China.
  • He Q; National Key Laboratory of Agricultural Microbiology, Wuhan 430070, Hubei, China.
  • Li W; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, Hubei, China.
Sheng Wu Gong Cheng Xue Bao ; 40(7): 1981-1996, 2024 Jul 25.
Article en Zh | MEDLINE | ID: mdl-39044570
ABSTRACT
Proteins serve as the primary executors of cellular activities in organisms, and thus investigating the subcellular localization and interactions of proteins is crucial for understanding protein functions and elucidating the molecular mechanisms in organisms. Proximity labeling is a recently developed effective method for detecting protein-protein interactions in live cells. Compared with the conventional methods for studying protein-protein interactions, proximity labeling demonstrates high sensitivity, strong specificity, and low background and is widely employed in the research of protein-protein interactions between pathogens and hosts. This article reviews the recent progress in the development and applications of the biotin ligase BirA and its mutants and elucidates the functioning principles of several classical biotin ligases. This review aims to clarify the role of proximity labeling based on BirA and its mutants in identifying protein-protein interactions between pathogens and hosts.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ligasas de Carbono-Nitrógeno / Interacciones Huésped-Patógeno / Mutación Límite: Humans Idioma: Zh Revista: Sheng Wu Gong Cheng Xue Bao Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Ligasas de Carbono-Nitrógeno / Interacciones Huésped-Patógeno / Mutación Límite: Humans Idioma: Zh Revista: Sheng Wu Gong Cheng Xue Bao Asunto de la revista: BIOTECNOLOGIA Año: 2024 Tipo del documento: Article País de afiliación: China Pais de publicación: China