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Analysis of N6-Methyladenosine Modification of HBV RNA by Methylated RNA Immunoprecipitation.
Kim, Geon-Woo; Siddiqui, Aleem.
Afiliación
  • Kim GW; Divsion of Infectious Diseases and Global Public Health, Department of Medicine, University of California, San Diego, La Jolla, CA, USA. gwkim3142@cnu.ac.kr.
  • Siddiqui A; Department of Microbiology and Molecular Biology, Chungnam National University, Yuseong-gu, Daejeon, Republic of Korea. gwkim3142@cnu.ac.kr.
Methods Mol Biol ; 2837: 59-66, 2024.
Article en En | MEDLINE | ID: mdl-39044075
ABSTRACT
Of all the chemical modifications of RNAs, the N6-methyladenosine (m6A) modification is the most prevalent and well-characterized RNA modification that is functionally implicated in a wide range of biological processes. The m6A modification occurs in hepatitis B virus (HBV) RNAs and this modification regulates the HBV life cycle in several ways. Thus, understanding the mechanisms underlying m6A modification of HBV RNAs is crucial in understanding HBV infectious process and associated pathogenesis. Here, we describe the currently utilized method in the detection and characterization of m6A-methylated RNAs during viral infection.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Viral / Adenosina / Virus de la Hepatitis B / Inmunoprecipitación Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: ARN Viral / Adenosina / Virus de la Hepatitis B / Inmunoprecipitación Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Estados Unidos Pais de publicación: Estados Unidos