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ADAMTS16 Suppression by MicroRNA-25 Leads to Oncogenic Properties in Renal Cell Carcinoma.
Huang, Jian; Tang, Zhe; Zhong, Guangjun; Guo, Linjie; Feng, Ye.
Afiliación
  • Huang J; Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, Hubei Province, P.R.China.
  • Tang Z; Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, Hubei Province, P.R.China.
  • Zhong G; Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, Hubei Province, P.R.China.
  • Guo L; Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, Hubei Province, P.R.China.
  • Feng Y; Department of Urology, The First Affiliated Hospital of Yangtze University, Jingzhou, 434000, Hubei Province, P.R.China.
Comb Chem High Throughput Screen ; 2024 Jul 22.
Article en En | MEDLINE | ID: mdl-39041274
ABSTRACT

INTRODUCTION:

Increasing evidence indicates that microRNAs (miRNAs) play a crucial role in modulating tumor growth. This study is centered on investigating the contribution of miR-25 to the progression of Renal Cell Carcinoma (RCC).

METHODS:

The investigators examined the expression levels of miR-25 and ADAMTS16 in RCC samples and cell lines. The association between miR-25 and ADAMTS16 was validated via a luciferase reporter assay. Cell viability, apoptosis, migration, and invasion were evaluated utilizing CCK-8 and flow cytometry techniques, while the expression levels of ADAMTS16, ß-catenin, GSK-3ß, and p-GSK-3ß were assessed through western blot analysis.

RESULTS:

The investigation revealed elevated expression levels of miR-25 in RCC tissues. Subsequently, ADAMTS16 was identified as a target of miR-25. Increased miR-25 levels were associated with decreased expression of ADAMTS16, resulting in enhanced cell viability and diminished apoptosis. Conversely, inhibition of miR-25 led to decreased cell viability, proliferation, and migration. Additionally, the researchers observed that miR-25 triggered the phosphorylation of GSK-3ß and ß-catenin while leaving the total GSK-3ß level unaffected.

CONCLUSION:

This study suggests that miR-25 regulates the expression of ADAMTS16 through the Wnt/ß-catenin signaling pathway, providing new insights into the cause and potential treatment of RCC.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Comb Chem High Throughput Screen Asunto de la revista: BIOLOGIA MOLECULAR / QUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Emiratos Árabes Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Idioma: En Revista: Comb Chem High Throughput Screen Asunto de la revista: BIOLOGIA MOLECULAR / QUIMICA Año: 2024 Tipo del documento: Article Pais de publicación: Emiratos Árabes Unidos