Identification of RVFV Host Factors Using Quantitative Interaction Proteomics.

Wilken, Lucas; Lasswitz, Lisa; Scaturro, Pietro; Gerold, Gisa

Wilken, Lucas; Lasswitz, Lisa; Scaturro, Pietro; Gerold, Gisa.

Afiliación

Wilken L; Leibniz Institute of Virology (LIV), Hamburg, Germany.
Lasswitz L; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany.
Scaturro P; Leibniz Institute of Virology (LIV), Hamburg, Germany. pietro.scaturro@leibniz-liv.de.
Gerold G; Institute of Biochemistry & Research Center for Emerging Infections and Zoonoses (RIZ), University of Veterinary Medicine Hannover, Hannover, Germany. gisa.gerold@tiho-hannover.de.

Methods Mol Biol ; 2824: 189-202, 2024.

Article en En | MEDLINE | ID: mdl-39039414

ABSTRACT

ABSTRACT

Affinity enrichment coupled with liquid chromatography-tandem mass spectrometry (AE-LC-MS/MS) enables a comprehensive study of virus-host protein-protein interactions in cells and tissues infected with Rift Valley fever virus (RVFV) or ectopically expressing RVFV proteins. Depending on the research question, different experimental setups with carefully chosen controls are needed. Here, we describe the detailed workflow of sample preparation, processing, and cleanup, while also outlining critical points to consider when designing and performing AE-LC-MS/MS experiments.

Asunto(s)

Interacciones Huésped-Patógeno; Proteómica; Virus de la Fiebre del Valle del Rift; Espectrometría de Masas en Tándem; Proteómica/métodos; Espectrometría de Masas en Tándem/métodos; Cromatografía Liquida/métodos; Humanos; Proteínas Virales/metabolismo; Fiebre del Valle del Rift/virología; Fiebre del Valle del Rift/metabolismo; Animales

Palabras clave

Affinity enrichment; Bottom-up proteomics; Immunoprecipitation; Mass spectrometry; Protein; Protein purification; Virus; host interaction; protein interaction

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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Virus de la Fiebre del Valle del Rift / Proteómica / Espectrometría de Masas en Tándem / Interacciones Huésped-Patógeno Límite: Animals / Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Alemania Pais de publicación: Estados Unidos

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