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Inverse Regulation of C-C Chemokine Receptor 3 Oligomerization by Downstream Proteins Indicates Biased Signal Transduction Pathways.
Li, Jiqiang; Li, Yu; Ding, Yanzhi; Song, Yanzhuo; Li, Junfeng; Chen, Haitao; Feng, Guoqing; Wang, Xiaojuan; Ge, Baosheng; Ding, Ning; Huang, Fang.
Afiliación
  • Li J; State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 266580, P. R. China.
  • Li Y; Qingdao Haier Biomedical Co., Ltd., Qingdao, Shandong 266000, P. R. China.
  • Ding Y; State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 266580, P. R. China.
  • Song Y; State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 266580, P. R. China.
  • Li J; State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 266580, P. R. China.
  • Chen H; Qingdao Haier Biomedical Co., Ltd., Qingdao, Shandong 266000, P. R. China.
  • Feng G; Qingdao Haier Biomedical Co., Ltd., Qingdao, Shandong 266000, P. R. China.
  • Wang X; Qingdao Haier Biomedical Co., Ltd., Qingdao, Shandong 266000, P. R. China.
  • Ge B; State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 266580, P. R. China.
  • Ding N; State Key Laboratory of Heavy Oil Processing and Center for Bioengineering and Biotechnology, China University of Petroleum (East China), Qingdao, Shandong 266580, P. R. China.
  • Huang F; Qingdao Huangdao District Hospital, Qingdao, Shandong 266580, P. R. China.
J Phys Chem Lett ; 15(30): 7652-7658, 2024 Aug 01.
Article en En | MEDLINE | ID: mdl-39037351
ABSTRACT
Oligomerization is one of the important mechanisms for G protein-coupled receptors (GPCRs) to modulate their activity in signal transduction. However, details of how and why the oligomerization of GPCRs regulates their functions under physiological conditions remain largely unknown. Here, using single-molecule photobleaching technology, we show that chemokine ligand 5 (CCL5) and chemokine ligand 8 (CCL8) are similar to the previously reported chemokine ligand 11 (CCL11) and chemokine ligand 24 (CCL24), which can regulate the oligomerization of chemokine receptor 3 (CCR3). Our results further demonstrate that downstream proteins, ß-arrestin 2 and Gi protein complex, on the CCR3 signal transduction pathway, can inversely regulate the oligomeric states of CCR3 induced by its binding ligands. This unexpected discovery suggests complex relationships between the oligomeric behaviors of CCR3 and the components of ligands-CCR3-downstream proteins, reflecting the potentially functional impact of the oligomerization on the multiple activation pathways of GPCR, such as biased activation.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Receptores CCR3 / Multimerización de Proteína Límite: Humans Idioma: En Revista: J Phys Chem Lett Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Transducción de Señal / Receptores CCR3 / Multimerización de Proteína Límite: Humans Idioma: En Revista: J Phys Chem Lett Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos