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Surgical Bone Implantation Technique for Rat Tibia Models of Diabetes and Osteoporosis.
Alfaiate, David; Mas-Moruno, Carlos; Manuela, Cidália; Ustrell, Josep Maria; Camara, Juan Antonio; Ferrer, Marina; Manero, José Maria; Manzanares-Céspedes, Maria-Cristina.
Afiliación
  • Alfaiate D; Faculty of Medicine and Health Sciences, University of Barcelona; Department of Oral Surgery, Davallmed Clinic. Porto; david.alfaiate@clinicadavallmed.com.
  • Mas-Moruno C; Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya; Barcelona Research Centre in Multiscale Science and Engineering, Universitat Politècnica de Catalunya.
  • Manuela C; Department of Periodontology, Davallmed Clinic. Porto.
  • Ustrell JM; Department of Odontostomatology, University of Barcelona; Oral Health and Masticatory System Research Group at the Bellvitge Biomedical Research Institute (IDIBELL).
  • Camara JA; Preclinical imaging platform, Vall d'Hebron Research Institute, Barcelona; Preclinical Therapeutics Core, Helen Diller Family Comprehensive Cancer Center, University of California, San Francisco.
  • Ferrer M; Animal facility, Vall d'Hebron Research Institute, Barcelona; UCSF Gnotobiotics Core Facility, University of California, San Francisco.
  • Manero JM; Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Engineering, Universitat Politècnica de Catalunya; Barcelona Research Centre in Multiscale Science and Engineering, Universitat Politècnica de Catalunya.
  • Manzanares-Céspedes MC; Human Anatomy and Embryology Unit, Experimental Pathology and Therapeutics Department, Faculty of Medicine and Health Sciences, University of Barcelona.
J Vis Exp ; (209)2024 07 05.
Article en En | MEDLINE | ID: mdl-39037246
ABSTRACT
The rat has long served as a valuable animal model in implant dentistry and orthopedics, particularly in studying the interactions between biomaterials and bone tissue. The rat's tibia is frequently chosen due to its easy surgical access through thin tissue layers (skin and muscle) and the flattened shape of its medial face, facilitating the surgical insertion of intraosseous devices. Additionally, this model enables the induction of specific diseases, mimicking various clinical conditions to assess biological responses to different implant conditions like geometry, surface texture, or biological cues. However, despite its robust cortical structure, certain intraosseous devices may require adaptations in design and size for successful implantation. Therefore, establishing standardized surgical methods for manipulating both soft and hard tissues in the implantation region is essential for ensuring proper implant or screw device placement, particularly in fields like implant dentistry and orthopedics. This study included eighty Sprague Dawley rats divided into two groups based on their respective diseases Group 1 with osteoporosis and Group 2 with Type 2 Diabetes. Implantations were performed at 4 weeks and 12 weeks, with the same surgeon following a consistent surgical technique. A positive biological response was observed, indicating complete osseointegration of all implants placed. These results validate the success of the surgical protocol, which can be replicated for other studies and serve as a benchmark for the biomaterials community. Notably, osseointegration values remained stable at both 4 weeks and 12 weeks for both disease models, demonstrating a durable integration of the implant over time and emphasizing the establishment of an intimate bone connection as early as 4 weeks.
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Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoporosis / Tibia / Ratas Sprague-Dawley Límite: Animals Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos
Texto completo
Añadir a Mi BVS
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Base de datos: MEDLINE Asunto principal: Osteoporosis / Tibia / Ratas Sprague-Dawley Límite: Animals Idioma: En Revista: J Vis Exp Año: 2024 Tipo del documento: Article Pais de publicación: Estados Unidos